Cone-rod homeobox, Polyclonal Antibody

CRX; cone-rod homeobox; CORD2; CRD; LCA7; OTX3; cone-rod homeobox protein; orthodenticle homeobox 3; CRX antibody; cone-rod homeobox antibody; CORD2 antibody; CRD antibody; LCA7 antibody; OTX3 antibody; cone-rod homeobox protein antibody; orthodenticle homeobox 3 antibody; Cone-rod homeobox; Cone-rod homeobox (C Terminus)

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-CRX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 30kDa band observed in Rat Retina lysates (calculated MW of 32.3kDa according to Human NP_000545.1 and to Rat NP_068627.1). Recommended concentration: 1-3ug/ml.

(1ug/ml) staining of Rat Retina lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

32,261 Da

cone-rod homeobox

cone-rod homeobox protein

Cone-rod homeobox protein

CORD2  [Similar Products]

CRX_HUMAN

The protein encoded by this gene is a photoreceptor-specific transcription factor which plays a role in the differentiation of photoreceptor cells. This homeodomain protein is necessary for the maintenance of normal cone and rod function. Mutations in this gene are associated with photoreceptor degeneration, Leber congenital amaurosis type III and the autosomal dominant cone-rod dystrophy 2. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

CRX: Binds and transactivates the sequence 5'-TAATC[CA]-3' which is found upstream of several photoreceptor-specific genes, including the opsin genes. Acts synergistically with other transcription factors, e.g. NRL and RX, to regulate photoreceptor cell-specific gene transcription. Essential for the maintenance of mammalian photoreceptors. Defects in CRX are the cause of Leber congenital amaurosis type 7 (LCA7). LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children. Defects in CRX are the cause of cone-rod dystrophy type 2 (CORD2); also known as cone-rod retinal dystrophy 2 (CRD2). CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa. Defects in CRX are a cause of retinitis pigmentosa (RP). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the paired homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleus

Molecular Function: leucine zipper domain binding; nuclear hormone receptor binding; protein binding; transcription factor activity

Biological Process: circadian rhythm; organ morphogenesis; positive regulation of photoreceptor cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to stimulus; transcription from RNA polymerase II promoter; visual perception

Disease: Cone-rod Dystrophy 2; Leber Congenital Amaurosis 7; Retinitis Pigmentosa

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