Full Product Name
ACPT siRNA (Human)
Product Synonym Names
Testicular acid phosphatase
Product Gene Name
ACPT sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9BZG2
Specificity
ACPT siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ACPT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ACPT sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ACPT sirna
siRNA to inhibit ACPT expression using RNA interference
Applications Tested/Suitable for ACPT sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ACPT. It may not necessarily be applicable to this product.
NCBI Accession #
NP_149059.1
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NCBI GenBank Nucleotide #
NM_033068.2
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UniProt Primary Accession #
Q9BZG2
[Other Products]
UniProt Secondary Accession #
Q9BZG3; Q9BZG4; C0H3P7[Other Products]
UniProt Related Accession #
Q9BZG2[Other Products]
Molecular Weight
9,912 Da
NCBI Official Full Name
testicular acid phosphatase
NCBI Official Synonym Full Names
acid phosphatase, testicular
NCBI Official Symbol
ACPT [Similar Products]
NCBI Protein Information
testicular acid phosphatase
UniProt Protein Name
Testicular acid phosphatase
Protein Family
Testicular acid phosphatase
UniProt Gene Name
ACPT [Similar Products]
UniProt Entry Name
PPAT_HUMAN
NCBI Summary for ACPT
Acid phosphatases are enzymes capable of hydrolyzing orthophosphoric acid esters in an acid medium. This gene is up-regulated by androgens and is down-regulated by estrogens in the prostate cancer cell line. This gene exhibits a lower level of expression in testicular cancer tissues than in normal tissues. The protein encoded by this gene has structural similarity to prostatic and lysosomal acid phosphatases. Alternatively spliced transcript variants have been described, but their
biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for ACPT
ACPT: Dephosphorylates receptor tyrosine-protein kinase erbB-4 and inhibits the ligand-induced proteolytic cleavage. Belongs to the histidine acid phosphatase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.2; Membrane protein, integral; Motility/polarity/chemotaxis; Phosphatase (non-protein)
Chromosomal Location of Human Ortholog: 19q13.4
Cellular Component: integral to membrane
Molecular Function: acid phosphatase activity
Biological Process: dephosphorylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
