14-3-3 gamma, Recombinant Protein

Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein gamma polypeptide; YWHAG; 14-3-3 gamma,Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; gamma polypeptide; Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein; gamma polypeptide 14 3 3 protein gamma; 14 3 3 protein gamma subtype; 14 3 3gamma; 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide; KCIP 1; KCIP1; Protein kinase C inhibitor protein 1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide.

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 95% by SDS-PAGE

Liquid in phosphate buffered saline (pH 7.4), 10% glycerol

1 mg/ml (determined by Bradford assay) (lot specific)

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of 14-3-3 gamma recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3gamma, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. Recombinant human YWHAG was expressed in E Coli and purified by using conventional chromatography techniques.

Signal Transduction

SDS-PAGE

28 kDa (247 aa), confirmed by MALDI-TOF.

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma

14-3-3 protein gamma

14-3-3 protein gamma

KCIP-1  [Similar Products]

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Chen XQ, et al. (2005) Glia. 42 (4):315-24 ; Chen XQ, Yu AC, et al. (2002) Biochem Biophys Res Commun. 296 (3):657-63 ;

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