ATP synthase protein 8, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Guinea pig MT-ATP8. No significant cross-reactivity or interference between Guinea pig MT-ATP8 and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of MT-ATP8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS283080 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ATP synthase protein 8 (MT-ATP8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MT-ATP8. The ELISA analytical biochemical technique of the MBS283080 kit is based on MT-ATP8 antibody-MT-ATP8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MT-ATP8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MT-ATP8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MT-ATP8 in samples. An antibody specific for MT-ATP8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT-ATP8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT-ATP8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT-ATP8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

7,947 Da

ATP synthase F0 subunit 8

ATP synthase protein 8

ATP8; ATPASE8; MTATP8  [Similar Products]

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane ().

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