Product Name
Granulysin (GNLY), Polyclonal Antibody
Full Product Name
Granulysin (Biotin)
Product Synonym Names
Anti -Granulysin (Biotin)
Product Gene Name
anti-GNLY antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 2; NC_000002.11 (85921414..85925875). Location: 2p11.2
3D Structure
ModBase 3D Structure for P22749
Specificity
Recognizes human Granulysin. Cross-reactivity: <1% with rhNKG-2D.
Purity/Purification
Affinity Purified
Purified by human Granulysin affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS and BSA. Labeled with Biotin. Reconstitute with 1ml 0.1% BSA in TBS to a concentration of 0.05mg/ml.
Immunogen
NS0-derived recombinant human rhGranulysin.
Hybridoma
Produced in goat immunized with purified, NS0-derived, recombinant human Granulysin.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-GNLY antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-GNLY antibody
Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-GNLY antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-GNLY antibody
Suitable for use in Western blot and Flow cytometry., , Recommended Dilutions:, Western blot: 0.1-0.2ug/ml The detection limit is ~5 ng/lane under non-reducing and 20ng/lane under reducing conditions. In this format, this, antibody shows less than 1% cross-reactivity with rhNKG-2D., Flow cytometry: 25ug/ml and add 10il of the diluted solution to 1-2.5 x 10e5 cells. Total reaction volume should not exceed 200ul. Use PMA plus Ca 2+ ionomycin-activated PBMC., Optimal dilutions to be determined by the researcher.
NCBI/Uniprot data below describe general gene information for GNLY. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036615.2
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NCBI GenBank Nucleotide #
NM_012483.2
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UniProt Primary Accession #
P22749
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UniProt Secondary Accession #
P09325; Q6GU08[Other Products]
UniProt Related Accession #
P22749[Other Products]
Molecular Weight
16,374 Da[Similar Products]
NCBI Official Full Name
granulysin isoform 519
NCBI Official Synonym Full Names
granulysin
NCBI Official Symbol
GNLY [Similar Products]
NCBI Official Synonym Symbols
519; LAG2; NKG5; LAG-2; D2S69E; TLA519
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NCBI Protein Information
granulysin; lymphokine LAG-2; OTTHUMP00000160682; OTTHUMP00000203266; OTTHUMP00000229037; OTTHUMP00000229039; lymphocyte-activation gene 2; T-cell activation protein 519; T-lymphocyte activation gene 519
UniProt Protein Name
Granulysin
UniProt Synonym Protein Names
Lymphokine LAG-2; Protein NKG5; T-cell activation protein 519
Protein Family
Granulysin
UniProt Gene Name
GNLY [Similar Products]
UniProt Synonym Gene Names
LAG2; NKG5; TLA519 [Similar Products]
UniProt Entry Name
GNLY_HUMAN
NCBI Summary for GNLY
The product of this gene is a member of the saposin-like protein (SAPLIP) family and is located in the cytotoxic granules of T cells, which are released upon antigen stimulation. This protein is present in cytotoxic granules of cytotoxic T lymphocytes and natural killer cells, and it has antimicrobial activity against M. tuberculosis and other organisms. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
UniProt Comments for GNLY
GNLY: Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 2p11.2
Cellular Component: extracellular space
Biological Process: killing of cells of another organism; defense response to bacterium; cellular defense response; defense response to fungus
Research Articles on GNLY
1. Cytotoxic T- and natural killer (NK) cell-delivered transgenic granulysin induces endoplasmic reticulum stress, activating caspase-7 and distinct apoptotic pathways in target cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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