Cytochrome P450 27A1, Polyclonal Antibody

25-hydroxylase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; CP27A; Cytochrome P-450C27/25; Cytochrome P450 27, mitochondrial; Sterol 26-hydroxylase; Sterol 27-hydroxylase; Vitamin D

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Cytochrome P450 27A1 Antibody detects endogenous levels of total Cytochrome P450 27A1 protein.

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1 mg/ml (lot specific)

Stable at -20 degree C for at least 1 year.

Small volumes of anti-CYP27A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), ELISA (EIA)

WB: 1:500~1:1000
ELISA: 1:10000

Western blot analysis of lysates from HeLa cells, using Cytochrome P450 27A1 Antibody. The lane on the right is blocked with the synthesized peptide.

60 kDa

cytochrome P450 family 27 subfamily A member 1

sterol 26-hydroxylase, mitochondrial

Sterol 26-hydroxylase, mitochondrial

CYP27  [Similar Products]

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq, Jul 2008]

Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.

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