Product Name
PRPS1, siRNA
Full Product Name
PRPS1 siRNA (Mouse)
Product Synonym Names
Ribose-phosphate pyrophosphokinase 1; Phosphoribosyl pyrophosphate synthase I; PRS-I
Product Gene Name
PRPS1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9D7G0
Specificity
PRPS1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PRPS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PRPS1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PRPS1 sirna
siRNA to inhibit PRPS1 expression using RNA interference
Applications Tested/Suitable for PRPS1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PRPS1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_067438.1
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NCBI GenBank Nucleotide #
NM_021463.4
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UniProt Primary Accession #
Q9D7G0
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UniProt Secondary Accession #
Q76MX9[Other Products]
UniProt Related Accession #
Q9D7G0[Other Products]
Molecular Weight
34,834 Da
NCBI Official Full Name
ribose-phosphate pyrophosphokinase 1
NCBI Official Synonym Full Names
phosphoribosyl pyrophosphate synthetase 1
NCBI Official Symbol
Prps1 [Similar Products]
NCBI Official Synonym Symbols
PRS-I; C76571; C76678; Prps-1; 2310010D17Rik
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NCBI Protein Information
ribose-phosphate pyrophosphokinase 1
UniProt Protein Name
Ribose-phosphate pyrophosphokinase 1
UniProt Synonym Protein Names
Phosphoribosyl pyrophosphate synthase I; PRS-I
UniProt Gene Name
Prps1 [Similar Products]
UniProt Synonym Gene Names
PRS-I [Similar Products]
UniProt Entry Name
PRPS1_MOUSE
UniProt Comments for PRPS1
PRPS1: Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis. Defects in PRPS1 are the cause of phosphoribosylpyrophosphate synthetase superactivity (PRPS1 superactivity); also known as PRPS-related gout. It is a familial disorder characterized by excessive purine production, gout and uric acid urolithiasis. Defects in PRPS1 are the cause of Charcot-Marie-Tooth disease X-linked recessive type 5 (CMTX5); also known as optic atrophy-polyneuropathy-deafness or Rosenberg-Chutorian syndrome. CMTX5 is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies characterized by severely reduced motor nerve conduction velocities (NCVs) (less than 38m/s) and segmental demyelination and remyelination, and primary peripheral axonal neuropathies characterized by normal or mildly reduced NCVs and chronic axonal degeneration and regeneration on nerve biopsy. Defects in PRPS1 are the cause of ARTS syndrome (ARTS); also known as fatal ataxia X-linked with deafness and loss of vision. ARTS is a disorder characterized by mental retardation, early-onset hypotonia, ataxia, delayed motor development, hearing impairment, and optic atrophy. Susceptibility to infections, especially of the upper respiratory tract, can result in early death. Defects in PRPS1 are the cause of deafness X-linked type 1 (DFNX1); also known as congenital sensorineural deafness X-linked 2 (DFN2). It is a form of deafness characterized by progressive, severe-to-profound sensorineural hearing loss in males. Females manifest mild to moderate hearing loss. Belongs to the ribose-phosphate pyrophosphokinase family.
Protein type: Kinase, other; Nucleotide Metabolism - purine; EC 2.7.6.1; Carbohydrate Metabolism - pentose phosphate pathway
Cellular Component: protein complex
Molecular Function: transferase activity; ribose phosphate diphosphokinase activity; GDP binding; protein homodimerization activity; metal ion binding; nucleotide binding; magnesium ion binding; kinase activity; ADP binding; carbohydrate binding; ATP binding; AMP binding
Biological Process: AMP biosynthetic process; cellular biosynthetic process; nervous system development; ribose phosphate metabolic process; nucleotide biosynthetic process; hypoxanthine biosynthetic process; 5-phosphoribose 1-diphosphate biosynthetic process; ribonucleoside monophosphate biosynthetic process; purine nucleotide biosynthetic process; phosphorylation; purine base metabolic process
Research Articles on PRPS1
1. Only unedited pri-miR-376 RNAs were detected in the cochlea suggesting that the activity of PRPS1 in the inner ear may not be regulated through the editing of miR-376 cluster.
Precautions
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