Product Name
Lactoferrin (LTF), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Lactoferrin (LTF)
Product Gene Name
anti-LTF antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-V RWCVI STAEYSKCRQ WQSKIRRTNP MFCIRRASPT DCIRAIAAKR ADAVTLDGGL VFEADQYKLR PVAAEIYGTE ENPQTYYYAV AVVKKGFNFQ LNQLQGRKSC HTGLGRSAGW NIPIGLLRRF LDWAGPPEPL QKAVAKFFSQ SCVPCADGNA YPNLCQLCIG KGKDKCACSS QEPYFGYSGA FNCLHKGIGD VAFVKESTVF ENLPQKADRD KYELLCPDNT RKPVEAFREC HLARVPSHAV VARSVNGKEN SIWELLYQSQ KKFGKSNPQE FQLFGSPGQQ KDLLFRDATI GFLKIPSKID SKLYLGLPYL TAIQGLRE
3D Structure
ModBase 3D Structure for P14632
Species Reactivity
Porcine
Specificity
The antibody is a rabbit polyclonal antibody raised against LTF. It has been selected for its ability to recognize LTF in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant LTF (Val25~Glu348) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2047292
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customerâs specifications, please inquire.
Other Notes
Small volumes of anti-LTF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-LTF antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-LTF antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB) of anti-LTF antibody
Western Blot: Sample: Porcine Spleen lysate; Primary Ab: 1ug/ml Rabbit Anti-Porcine LTF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Western Blot (WB) of anti-LTF antibody
Western Blot: Sample: Recombinant protein.

NCBI/Uniprot data below describe general gene information for LTF. It may not necessarily be applicable to this product.
NCBI Accession #
NP_999527.1
[Other Products]
NCBI GenBank Nucleotide #
NM_214362.1
[Other Products]
UniProt Primary Accession #
P14632
[Other Products]
UniProt Secondary Accession #
Q29557[Other Products]
UniProt Related Accession #
P14632[Other Products]
Molecular Weight
77,626 Da
NCBI Official Full Name
lactotransferrin
NCBI Official Symbol
LTF [Similar Products]
NCBI Official Synonym Symbols
PLF
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NCBI Protein Information
lactotransferrin
UniProt Protein Name
Lactotransferrin
Protein Family
Lactotransferrin
UniProt Gene Name
LTF [Similar Products]
UniProt Synonym Gene Names
Lactoferrin [Similar Products]
UniProt Comments for LTF
Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate.Lactotransferrin is a major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. Has antimicrobial activity. Antimicrobial properties may include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. May have anabolic, differentiating and anti-apoptotic effects on osteoblasts and may also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth. May interfere with the lipopolysaccharide (LPS)-stimulated TLR4 signaling ().
Research Articles on LTF
1. this paper shows that recombinant lactoferrin peptide LF-6 produced with intein technology has both antibacterial and immunomodulatory activity in mice
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.