Sclerostin, ELISA Kit

Sclerostin

For Research Use Only. Not for use in diagnostic procedures.

The Rat Sclerostin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat Sclerostin proteins within the range of 15.6 pg/ml - 1000 pg/ml.

Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of SOST elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS8248486 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sclerostin (SOST) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SOST. The ELISA analytical biochemical technique of the MBS8248486 kit is based on SOST antibody-SOST antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SOST antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SOST. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: The Rat Sclerostin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Sclerostin in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Rat Sclerostin coated on a 96-well plate. Standards and samples are pipetted into the wells and Sclerostin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Sclerostin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Sclerostin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Sclerostin is a protein that in humans is encoded by the SOST gene. It is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Additionally, Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21.

23,974 Da

sclerostin

sclerostin

Sclerostin

SOST_RAT

may act as a regulator of bone homeostasis [RGD, Feb 2006]

SOST: Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular matrix; extracellular space; Golgi apparatus; protein complex

Molecular Function: protein binding; transcription factor binding

Biological Process: negative regulation of BMP signaling pathway; negative regulation of ossification; negative regulation of protein complex assembly; negative regulation of Wnt receptor signaling pathway; ossification; positive regulation of transcription, DNA-dependent; response to mechanical stimulus

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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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