Lactoferrin, ELISA Kit

Plasma Protein ELISA Kits, Human Kits

For Research Use Only. Not for use in diagnostic procedures.

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Small volumes of HLTFKT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS136073 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lactoferrin (HLTFKT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HLTFKT. The ELISA analytical biochemical technique of the MBS136073 kit is based on HLTFKT antibody-HLTFKT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HLTFKT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HLTFKT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Human lactoferrin (aka lactotransferrin or growth-inhibiting protein 12) is an 82.4kDa 691 amino acid glycoprotein which carries 2 ferric ions per molecule and is the main iron binding protein in milk. Lactoferrin has multiple physiological functions including iron absorption regulation and antibacterial, antiviral, and antiparasitic activity. Degranulated neutrophils are the primary source of plasma lactoferrin which is increased during inflammation and bacterial infection. The sensitive quantitative measurement of human lactoferrin in human plasma, serum, milk, and cell culture samples is easily performed with this 96 well strip format ELISA kit. The normal human concentration of lactoferrin is 100 ng/ml in plasma and 5.3 mg/ml in milk. The assay measures human lactoferrin in the 0.1-100 ng/ml range. Samples giving human lactoferrin levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:100-1:500 dilution for plasma or serum and 1:200,000 dilution for breast milk is suggested for best results. Human lactoferrin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated anti-human lactoferrin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared using dilutions of human lactoferrin and is measured along with the test samples. Color development is proportional to the concentration of total lactoferrin in the samples. All reagents and standards are provided in these ELISA kits.

HUMAN PLASMA PROTEINS

73,161 Da

lactotransferrin

lactotransferrin

Lactotransferrin

GIG12; LF; Lactoferrin; Lfcin-H  [Similar Products]

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate.

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