Full Product Name
OPA1 Antibody
Product Synonym Names
Dynamin-like 120 kDa protein, mitochondrial; EC 3.6.5.5; Optic atrophy protein 1; [Cleaved into: Dynamin-like 120 kDa protein; form S1]; OPA1; KIAA0567
Product Gene Name
anti-OPA1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O60313
Species Reactivity
Human, Mouse
Purity/Purification
Antigen Affinity Purified
Immunogen
Recombinant Human Dynamin-like 120 kDa protein, mitochondrial protein (183-351AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-OPA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-OPA1 antibody
Neuroscience
Applications Tested/Suitable for anti-OPA1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-OPA1 antibody
WB: 1:1000-1:5000
Western Blot (WB) of anti-OPA1 antibody
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Mouse heart tissue
All lanes: OPA1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 112, 116 kDa
Observed band size: 112 kDa
NCBI/Uniprot data below describe general gene information for OPA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_056375.2
[Other Products]
NCBI GenBank Nucleotide #
NM_015560.2
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UniProt Primary Accession #
O60313
[Other Products]
UniProt Secondary Accession #
D3DNW4; E5KLJ5; E5KLJ6; E5KLJ7; E5KLK1; E5KLK2[Other Products]
UniProt Related Accession #
O60313[Other Products]
Molecular Weight
115,884 Da
NCBI Official Full Name
dynamin-like 120 kDa protein, mitochondrial isoform 1 preproprotein
NCBI Official Synonym Full Names
OPA1, mitochondrial dynamin like GTPase
NCBI Official Symbol
OPA1 [Similar Products]
NCBI Official Synonym Symbols
NPG; NTG; MGM1; BERHS; largeG; MTDPS14
[Similar Products]
NCBI Protein Information
dynamin-like 120 kDa protein, mitochondrial
UniProt Protein Name
Dynamin-like 120 kDa protein, mitochondrial
UniProt Synonym Protein Names
Optic atrophy protein 1
Protein Family
Dynamin-like 120 kDa protein
UniProt Gene Name
OPA1 [Similar Products]
NCBI Summary for OPA1
The protein encoded by this gene is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. The encoded protein localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. [provided by RefSeq, Aug 2017]
UniProt Comments for OPA1
Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission (PubMed:16778770, PubMed:17709429, PubMed:20185555, PubMed:24616225, PubMed:28746876). Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion (PubMed:17709429). Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation (PubMed:20185555). The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes (PubMed:20185555). Plays a role in remodeling cristae and the release of cytochrome c during apoptosis (). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space (). Plays a role in mitochondrial genome maintenance (PubMed:20974897, PubMed:18158317).
Research Articles on OPA1
1. Our data indicate that the LEU396ARG mutation in OPA1 is associated with severe dominant optic atrophy.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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