Full Product Name
NF1 siRNA (Rat)
Product Synonym Names
Neurofibromin; Neurofibromatosis-related protein NF-1
Product Gene Name
NF1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P97526
Specificity
NF1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat NF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of NF1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
NF1 sirna
siRNA to inhibit NF1 expression using RNA interference
Applications Tested/Suitable for NF1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for NF1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036741.1
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NCBI GenBank Nucleotide #
NM_012609.1
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UniProt Primary Accession #
P97526
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UniProt Related Accession #
P97526[Other Products]
Molecular Weight
317,083 Da
NCBI Official Full Name
neurofibromin
NCBI Official Synonym Full Names
neurofibromin 1
NCBI Official Symbol
Nf1 [Similar Products]
NCBI Protein Information
neurofibromin
UniProt Protein Name
Neurofibromin
UniProt Synonym Protein Names
Neurofibromatosis-related protein NF-1
Protein Family
Neurofibromin
UniProt Gene Name
Nf1 [Similar Products]
UniProt Entry Name
NF1_RAT
NCBI Summary for NF1
mutations in gene are associated with neurofibromatosis type 1 [RGD, Feb 2006]
UniProt Comments for NF1
NF1: a Ras-GAP, highly expressed in developing neural cells. Possesses tumor suppressor activity, presumably by virtue of its GTPase activating domain. Neurofibromin is phosphorylated in response to EGF in CNS cells and cell lines. Defects in NF1 are the cause of type 1 neurofibromatosis (NF1), Watson syndrome, and familial spinal neurofibromatosis. NF1 is one of the most frequent autosomal dominant diseases. Four alternatively spliced isoforms have been described.
Protein type: Motility/polarity/chemotaxis; Nucleolus; Tumor suppressor; GAPs, Ras; GAPs
Cellular Component: neuron projection; protein complex; membrane; axon; dendrite; postsynaptic density; cytoplasm; nucleolus; intracellular; nucleus
Molecular Function: syndecan binding; protein binding; phosphatidylethanolamine binding; microtubule binding; phosphatidylcholine binding; GTPase activator activity
Biological Process: negative regulation of Rac protein signal transduction; neural tube development; negative regulation of MAP kinase activity; Schwann cell development; extracellular matrix organization and biogenesis; collagen fibril organization; regulation of neuron differentiation; positive regulation of apoptosis; heart development; negative regulation of transcription factor import into nucleus; negative regulation of neuroblast proliferation; regulation of glial cell differentiation; positive regulation of adenylate cyclase activity; negative regulation of neurotransmitter secretion; skeletal muscle development; adrenal gland development; negative regulation of cell-matrix adhesion; forebrain morphogenesis; regulation of synaptic transmission, GABAergic; myelination in the peripheral nervous system; camera-type eye morphogenesis; induction of apoptosis via death domain receptors; negative regulation of fibroblast proliferation; negative regulation of endothelial cell proliferation; negative regulation of protein kinase activity; positive regulation of endothelial cell proliferation; cerebral cortex development; actin cytoskeleton organization and biogenesis; forebrain astrocyte development; metanephros development; regulation of long-term neuronal synaptic plasticity; negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; wound healing; regulation of cell-matrix adhesion; regulation of blood vessel endothelial cell migration; smooth muscle development; negative regulation of cell proliferation; sympathetic nervous system development; positive regulation of neuron apoptosis; visual learning; negative regulation of Ras protein signal transduction; cell communication; negative regulation of cell migration; regulation of bone resorption; negative regulation of MAPKKK cascade; MAPKKK cascade; liver development; regulation of angiogenesis; peripheral nervous system development; regulation of cell proliferation; negative regulation of oligodendrocyte differentiation; osteoblast differentiation; negative regulation of angiogenesis; pigmentation; spinal cord development; Ras protein signal transduction; negative regulation of astrocyte differentiation; response to hypoxia; artery morphogenesis; brain development; cognition
Research Articles on NF1
1. Ras inactivation by neurofibromin is required for sustained spine enlargement associated with long term potentiation (LTP) and maintenance of spine density.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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