Product Name
ADH1B, siRNA
Full Product Name
ADH1B siRNA (Human)
Product Synonym Names
ADH2; Alcohol dehydrogenase 1B; Alcohol dehydrogenase subunit beta
Product Gene Name
ADH1B sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P00325
Specificity
ADH1B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADH1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ADH1B sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ADH1B sirna
siRNA to inhibit ADH1B expression using RNA interference
Applications Tested/Suitable for ADH1B sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ADH1B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000659.2
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NCBI GenBank Nucleotide #
NM_000668.5
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UniProt Primary Accession #
P00325
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UniProt Secondary Accession #
Q13711; Q4ZGI9; Q96KI7; A8MYN5; B4DRS9; B4DVC3[Other Products]
UniProt Related Accession #
P00325[Other Products]
Molecular Weight
35,412 Da
NCBI Official Full Name
alcohol dehydrogenase 1B isoform 1
NCBI Official Synonym Full Names
alcohol dehydrogenase 1B (class I), beta polypeptide
NCBI Official Symbol
ADH1B [Similar Products]
NCBI Official Synonym Symbols
ADH2; HEL-S-117
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NCBI Protein Information
alcohol dehydrogenase 1B
UniProt Protein Name
Alcohol dehydrogenase 1B
UniProt Synonym Protein Names
Alcohol dehydrogenase subunit beta
UniProt Gene Name
ADH1B [Similar Products]
UniProt Synonym Gene Names
ADH2 [Similar Products]
UniProt Entry Name
ADH1B_HUMAN
NCBI Summary for ADH1B
The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
UniProt Comments for ADH1B
ADH1B: Dimer of identical or non-identical chains of three types; alpha, beta and gamma. Belongs to the zinc-containing alcohol dehydrogenase family.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - metabolism by cytochrome P450; Lipid Metabolism - fatty acid; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.1.1.1; Amino Acid Metabolism - tyrosine
Chromosomal Location of Human Ortholog: 4q23
Cellular Component: cytosol
Molecular Function: zinc ion binding; alcohol dehydrogenase activity, zinc-dependent
Biological Process: xenobiotic metabolic process; ethanol oxidation
Disease: Alcohol Dependence
Research Articles on ADH1B
1. The risk factor of best friends drinking attenuates the protective effect of a well-established ADH1B variant for 2 adolescent drinking behaviors.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
