Product Name
Protein four-jointed (fj), Recombinant Protein
Full Product Name
Recombinant Drosophila melanogaster Protein four-jointed (fj), partial
Product Gene Name
fj recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
100-583
Sequence
G LPRHQDSPPD LPEEQIQMVA VEPLSSYRVE FIKETDELSA EQVFRNAFHL EQDKDAPDSM VVKKLDTNDG SIKEFHVQRT ASGRYRKGPE RRLSKKMPER VQPQETSRSP TTSPTNPTSE HQAGFIEEDV YWGPTVEQAL PKGFAAKDQV SWERFVGEQG RVVRLEQGCG RMQNRMVVFA DGTRACARYR QNTDQIQGEI FSYYLGQLLN ISNLAPSAAT VVDTSTPNWA AALGDITQAQ WKERRPVVLT RWLSDLEPAG IPQPFQPLER HLNKHDVWNL TRHMQSERQA QSQPHGLLKR LGAASSPGSA HQSNAIEETG TGTETANGAL VQRLIELAQW SDLIVFDYLI ANLDRVVNNL YNFQWNADIM AAPAHNLARQ SASQLLVFLD NESGLLHGYR LLKKYEAYHS LLLDNLCVFR RPTIDALRRL RAAGAGRRLR DLFERTTSAG VRDVLPSLPD KSVKILVERI DRVLGQVQKC QGS
3D Structure
ModBase 3D Structure for P54360
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Drosophila melanogaster (Fruit fly)
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of fj recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for fj. It may not necessarily be applicable to this product.
NCBI Accession #
NP_477483.1
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NCBI GenBank Nucleotide #
NM_058135.4
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UniProt Primary Accession #
P54360
[Other Products]
UniProt Secondary Accession #
Q24176; Q8SZ37; Q9V8E3[Other Products]
UniProt Related Accession #
P54360[Other Products]
Molecular Weight
65,490 Da
NCBI Official Full Name
four-jointed
NCBI Official Synonym Full Names
four-jointed
NCBI Official Symbol
fj [Similar Products]
NCBI Official Synonym Symbols
CG10917; DmelCG10917; Fj
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NCBI Protein Information
CG10917 gene product from transcript CG10917-RA
UniProt Protein Name
Extracellular serine/threonine protein kinase four-jointed
UniProt Gene Name
fj [Similar Products]
UniProt Comments for fj
Golgi serine/threonine protein kinase required for intermediate growth in the proximal-distal axis. Phosphorylates specific residues within extracellular cadherin domains of Fat (ft) and Dachsous (ds) as they transit through the Golgi (PubMed:18635802). Acts in ommatidial polarity determination as a secondary signal downstream of Notch, JAK/STAT and wingless. Also necessary for the initiation, up-regulation or maintenance of Notch ligand, Serrate (Ser) expression in legs, thereby participating in a feedback loop with N signaling. Sufficient for joint formation and growth in the leg (PubMed:10607560, PubMed:11566858, PubMed:14757640, PubMed:7555705, PubMed:8606003).
Research Articles on fj
1. Four-jointed acts both on Fat to promote its binding to Dachsous and on Dachsous to inhibit its binding to Fat, with a bias towards a stronger effect on Fat
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
