Product Name
Lactoferrin (LTF), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Lactoferrin (LTF)
Product Gene Name
anti-LTF antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -DADAM SLDGGYIYTA GKCGLVPVLA ENQKSSKSNG LDCVNRPVEG YLAVAAVRRE DAGFTWSSLR GKKSCHTAVD RTAGWNIPMG LLANQTRSCK FNEFFSQSCA PGADPKSNLC ALCIGDEKGE NKCAPNSKER YQGYTGALR
3D Structure
ModBase 3D Structure for P08071
Specificity
The antibody is a rabbit polyclonal antibody raised against LTF. It has been selected for its ability to recognize LTF in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant LTF (Asp406~Arg549) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2047280
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customerâs specifications, please inquire.
Other Notes
Small volumes of anti-LTF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-LTF antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-LTF antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB) of anti-LTF antibody
Western Blot: Sample: Recombinant protein.

Immunohistochemistry (IHC) of anti-LTF antibody
DABstainingonIHC-P.Samples:MouseTissue)

NCBI/Uniprot data below describe general gene information for LTF. It may not necessarily be applicable to this product.
NCBI Accession #
NP_032548.2
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NCBI GenBank Nucleotide #
NM_008522.3
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UniProt Primary Accession #
P08071
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UniProt Secondary Accession #
P70690; Q61799; Q8CBA0; Q922P2[Other Products]
UniProt Related Accession #
P08071[Other Products]
Molecular Weight
77,838 Da
NCBI Official Full Name
lactotransferrin
NCBI Official Synonym Full Names
lactotransferrin
NCBI Official Symbol
Ltf [Similar Products]
NCBI Official Synonym Symbols
Lf; Csp82; Ms10r; MMS10R
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NCBI Protein Information
lactotransferrin
UniProt Protein Name
Lactotransferrin
Protein Family
Lactotransferrin
UniProt Gene Name
Ltf [Similar Products]
UniProt Synonym Gene Names
Lactoferrin [Similar Products]
UniProt Comments for LTF
LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.
Protein type: EC 3.4.21.-; Protease; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 9 F2|9 60.79 cM
Cellular Component: cell surface; cytoplasm; extracellular exosome; extracellular region; extracellular space; nucleus; other organism cell membrane; protein complex; secretory granule; specific granule
Molecular Function: cysteine-type endopeptidase inhibitor activity; heparin binding; hydrolase activity; iron ion binding; lipopolysaccharide binding; metal ion binding; peptidase activity; protein serine/threonine kinase activator activity; serine-type endopeptidase activity; serine-type peptidase activity
Biological Process: antibacterial humoral response; antifungal humoral response; antimicrobial humoral immune response mediated by antimicrobial peptide; defense response to Gram-negative bacterium; immune system process; innate immune response in mucosa; ion transport; iron ion homeostasis; killing of cells of other organism; membrane disruption in other organism; negative regulation by host of viral process; negative regulation of ATPase activity; negative regulation of cysteine-type endopeptidase activity; negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of single-species biofilm formation in or on host organism; negative regulation of viral genome replication; negative regulation of viral process; ossification; positive regulation of chondrocyte proliferation; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of NF-kappaB transcription factor activity; positive regulation of osteoblast differentiation; positive regulation of osteoblast proliferation; positive regulation of protein serine/threonine kinase activity; positive regulation of toll-like receptor 4 signaling pathway; proteolysis; regulation of cytokine production; regulation of tumor necrosis factor production; transport
Research Articles on LTF
1. Data indicate that lactoferrin uptake across duodenum by glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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