Full Product Name
Epo-R (Phospho-Tyr456) Antibody
Product Synonym Names
EPOR; EPO Receptor; EPO-R; Erythropoietin receptor
Product Gene Name
anti-Epo-R antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P19235
Species Reactivity
Human, Mouse, Rat
Specificity
Epo-R (Phospho-Tyr456) Antibody detects endogenous levels of Epo-R only when phosphorylated at Tyr456
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human Epo-R (Phospho-Tyr456)
Modification
Phospho-Tyr456
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-Epo-R antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-Epo-R antibody
Phospho-specific Ab
Applications Tested/Suitable for anti-Epo-R antibody
Western Blot (WB)
Application Notes for anti-Epo-R antibody
Western Blot: 1:1000
Western Blot (WB) of anti-Epo-R antibody
Western blot analysis Epo-R (Phospho-Tyr456) using Jurkat whole cell lysates
NCBI/Uniprot data below describe general gene information for Epo-R. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000112.1
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NCBI GenBank Nucleotide #
NM_000121.3
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UniProt Primary Accession #
P19235
[Other Products]
UniProt Secondary Accession #
Q15443; Q2M205; B2RCG4[Other Products]
UniProt Related Accession #
P19235[Other Products]
Molecular Weight
35,809 Da
NCBI Official Full Name
erythropoietin receptor
NCBI Official Synonym Full Names
erythropoietin receptor
NCBI Official Symbol
EPOR [Similar Products]
NCBI Official Synonym Symbols
EPO-R
[Similar Products]
NCBI Protein Information
erythropoietin receptor
UniProt Protein Name
Erythropoietin receptor
UniProt Gene Name
EPOR [Similar Products]
UniProt Synonym Gene Names
EPO-R [Similar Products]
UniProt Entry Name
EPOR_HUMAN
NCBI Summary for Epo-R
This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
UniProt Comments for Epo-R
EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 19p13.3-p13.2
Cellular Component: integral to plasma membrane
Molecular Function: erythropoietin receptor activity; identical protein binding; protein binding
Disease: Erythrocytosis, Familial, 1
Research Articles on Epo-R
1. In multivariate survival analysis, age, Epo and EpoR were independent prognostic factors related to overall survival in hepatocellular carcinoma.
Precautions
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Disclaimer
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