14-3-3, sigma, Monoclonal Antibody

Anti -14-3-3, sigma (SFN, Stratifin, HGNC:10773)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 1; NC_000001.10 (27189633..27190947). Location: 1p36.11

Monoclonal

IgG1,k

1.T.28

Mouse

Recognizes human 14-3-3 sigma, Mr 30kD. Does not crossreact with other 14-3-3 isoforms. Species Crossreactivity: rat and mouse.

Affinity Purified
Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, pH 7.6, 0.07% sodium azide, before the addition of glycerol to 30%.

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-SFN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also found in plants and fungi. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins which bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein.

Antibodies; Abs to 14-3-3

Western Blot (WB), Immunoprecipitation (IP)

Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 0.5-2ug/ml. EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with 0014-19. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

27,774 Da[Similar Products]

stratifin

14-3-3 protein sigma; 14-3-3 sigma; OTTHUMP00000004242; epithelial cell marker protein 1

14-3-3 protein sigma

HME1  [Similar Products]

1433S_HUMAN

14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: extracellular space; cytoplasmic vesicle membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein domain specific binding; protein kinase C inhibitor activity; protein binding; phosphoprotein binding; protein kinase binding

Biological Process: positive regulation of protein export from nucleus; release of cytochrome c from mitochondria; regulation of epidermal cell division; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; keratinization; positive regulation of epidermal cell differentiation; negative regulation of protein kinase activity; negative regulation of caspase activity; regulation of cyclin-dependent protein kinase activity; signal transduction; positive regulation of cell growth

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