Product Name
Avidin (AVD), Protein
Full Product Name
Avidin (Egg White, 68kD Glycoprotein)
Product Gene Name
AVD protein
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: Z; NC_006127.3 (9023591..9024771). Location: chromosome: Z
3D Structure
ModBase 3D Structure for P02701
Purity/Purification
Affinity Purified
Purified by affinity chromatographic techniques.
Form/Format
Supplied as a lyophilized powder.
Activity
Greater than 11 U/mg, 1 unit binds 1µg biotin.
Endotoxin Level
0.1ng/ug (IEU/ug) of Avidin.
Solubility
It is recommended to reconstitute the lyophilized Avidin in sterile 18M -cm H2O not less than 100µg/ml or more than 10mg/ml. solutions
Preparation and Storage
-20 degree C
Stability: Avidin although stable at 40C for 3 weeks, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Other Notes
Small volumes of AVD protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AVD protein
Avidin is a glycosylated polypeptide chain having a molecular mass of 68kD and containg 4 subunits each with a for biotin. Avidin is purified by affinity chromatographic techniques. The purification procedure ensures minimal contamination by other proteins or DNA. The resulting high activity and purity of the product gives very low non-specific binding (NSB).
Product Categories/Family for AVD protein
BioSeparation; BioSep-Assay Detection, Tags
Application Notes for AVD protein
Avidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques.
NCBI/Uniprot data below describe general gene information for AVD. It may not necessarily be applicable to this product.
NCBI Accession #
NP_990651.1
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NCBI GenBank Nucleotide #
NM_205320.1
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UniProt Primary Accession #
P02701
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UniProt Secondary Accession #
Q91958; Q98SH4[Other Products]
UniProt Related Accession #
P02701[Other Products]
Molecular Weight
68kD[Similar Products]
NCBI Official Full Name
avidin
NCBI Official Synonym Full Names
avidin
NCBI Official Symbol
AVD [Similar Products]
NCBI Protein Information
avidin; mature avidin
UniProt Protein Name
Avidin
UniProt Gene Name
AVD [Similar Products]
UniProt Entry Name
AVID_CHICK
UniProt Comments for AVD
Function: The
biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
Subunit structure: Homotetramer.
Subcellular location: Secreted.
Tissue specificity: Synthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).
Post-translational modification: N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.
Sequence similarities: Belongs to the avidin/streptavidin family.Contains 1 avidin-like domain.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
