Full Product Name
KRAS Antibody: ATTO 565
Product Synonym Names
Rabbit Anti-Human KRAS Polyclonal; Transforming protein p21 Antibody; c-Ki-ras Antibody; K RAS4A Antibody; v Ki ras2 Kirsten rat sarcoma viral oncogene homolog Antibody; PR310 cK ras oncogene Antibody; Oncogene KRAS2 Antibody; KRAS Antibody; KRAS1 Antibody; RASK2 Antibody; K RAS2A Antibody; K RAS p21 protein Antibody; GTPase KRas Antibody; c-K-ras Antibody; K-Ras 2 Antibody; KRAS2 Antibody; c Kirsten ras protein Antibody; K RAS2B Antibody; p21ras Antibody; Cellular c Ki ras2 proto oncogene Antibody; Kirsten rat sarcoma 2 viral (v Ki ras2) oncogene homolog Antibody; Ki-Ras Antibody; RASK_HUMAN Antibody; KI RAS Antibody; NS3 Antibody; K RAS4B Antibody; cK Ras Antibody; KIRSTEN MURINE SARCOMA VIRUS 2 Antibody; NS Antibody; N-terminally processed Antibody
Product Gene Name
anti-KRAS antibody
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Matching Pairs
Unconjugated Antibody: KRAS (MBS8001417)
ATTO 565 Conjugated Antibody: KRAS (MBS8001420)
Matching Pairs
Unconjugated Antibody: N/A (MBS8001420)
ATTO 565 Conjugated Antibody: KRAS (MBS8001420)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P01116
Specificity
Detects ~29 kDa. Band is higher than the predicted molecular weight due to post translational modifications.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Immunogen
Synthetic peptide from the C-terminal of Human KRAS (aa. 100-189)
Cellular Localization
Cell Membrane; Lipid-Anchor; Cytoplasmic Side; Cytoplasm; Cytosol
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Preparation and Storage
Store at -20 degree C.
Other Notes
Small volumes of anti-KRAS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-KRAS antibody
Antibodies; Cell Signaling; G Protein Signaling; Small G Proteins; Ras Family; Cell Signaling; Epigenetics and Nuclear Signaling; Transcription; Cancer
Applications Tested/Suitable for anti-KRAS antibody
Western Blot (WB)
Application Notes for anti-KRAS antibody
WB: 1:1000
Western Blot (WB) of anti-KRAS antibody
Western blot analysis of Rat Kidney showing detection of ~21.6 kDa KRAS protein using Rabbit Anti-KRAS Polyclonal Antibody (SPC-777). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-KRAS Polyclonal Antibody (SPC-777) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.6 kDa. Other Band(s): ~29 kDa (PTM).
NCBI/Uniprot data below describe general gene information for KRAS. It may not necessarily be applicable to this product.
NCBI Accession #
NP_004976.2
[Other Products]
NCBI GenBank Nucleotide #
NM_004985.4
[Other Products]
UniProt Primary Accession #
P01116
[Other Products]
UniProt Secondary Accession #
P01118; Q96D10; A8K8Z5; B0LPF9[Other Products]
UniProt Related Accession #
P01116[Other Products]
Molecular Weight
21,425 Da
NCBI Official Full Name
GTPase KRas isoform b
NCBI Official Synonym Full Names
KRAS proto-oncogene, GTPase
NCBI Official Symbol
KRAS [Similar Products]
NCBI Official Synonym Symbols
NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
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NCBI Protein Information
GTPase KRas
UniProt Protein Name
GTPase KRas
UniProt Synonym Protein Names
K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras
UniProt Gene Name
KRAS [Similar Products]
UniProt Synonym Gene Names
KRAS2; RASK2 [Similar Products]
NCBI Summary for KRAS
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
UniProt Comments for KRAS
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed:24623306).
Research Articles on KRAS
1. KRAS Polymorphism is associated with recurrence in non-small cell lung cancer.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
