Full Product Name
SGCD siRNA (Human)
Product Synonym Names
Delta-sarcoglycan; Delta-SG; 35 kDa dystrophin-associated glycoprotein; 35DAG
Product Gene Name
SGCD sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q92629
Specificity
SGCD siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SGCD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SGCD sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SGCD sirna
siRNA to inhibit SGCD expression using RNA interference
Applications Tested/Suitable for SGCD sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SGCD. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000328.2
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NCBI GenBank Nucleotide #
NM_000337.5
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UniProt Primary Accession #
Q92629
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UniProt Secondary Accession #
Q53XA5; Q99644; A8K9S9[Other Products]
UniProt Related Accession #
Q92629[Other Products]
Molecular Weight
28,750 Da
NCBI Official Full Name
delta-sarcoglycan isoform 1
NCBI Official Synonym Full Names
sarcoglycan, delta (35kDa dystrophin-associated glycoprotein)
NCBI Official Symbol
SGCD [Similar Products]
NCBI Official Synonym Symbols
SGD; DAGD; 35DAG; CMD1L; SGCDP; SG-delta
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NCBI Protein Information
delta-sarcoglycan
UniProt Protein Name
Delta-sarcoglycan
UniProt Synonym Protein Names
35 kDa dystrophin-associated glycoprotein; 35DAG
Protein Family
Delta-sarcoglycan
UniProt Gene Name
SGCD [Similar Products]
UniProt Synonym Gene Names
Delta-SG; 35DAG [Similar Products]
UniProt Entry Name
SGCD_HUMAN
NCBI Summary for SGCD
The protein encoded by this gene is one of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). DGC forms a link between the F-actin cytoskeleton and the extracellular matrix. This protein is expressed most abundantly in skeletal and cardiac muscle. Mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for SGCD
SGCD: Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. Defects in SGCD are the cause of limb-girdle muscular dystrophy type 2F (LGMD2F). LGMD2F is an autosomal recessive disorder. Defects in SGCD are the cause of cardiomyopathy dilated type 1L (CMD1L). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the sarcoglycan beta/delta/gamma/zeta family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Dystrophin complex; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5q33-q34
Cellular Component: dystrophin-associated glycoprotein complex; cytoskeleton; cytoplasm; integral to membrane; plasma membrane; sarcoglycan complex; sarcolemma
Biological Process: cardiac muscle development; muscle development; heart contraction; muscle cell development
Disease: Muscular Dystrophy, Limb-girdle, Type 2f; Cardiomyopathy, Dilated, 1l
Research Articles on SGCD
1. CC genotype of the delta-sarcoglycan gene polymorphism rs13170573 is associated with obstructive sleep apnea in the Chinese population
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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