Full Product Name
HAMP siRNA (Rat)
Product Synonym Names
HEPC; Hepcidin
Product Gene Name
HAMP sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q99MH3
Specificity
HAMP siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat HAMP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of HAMP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HAMP sirna
siRNA to inhibit HAMP expression using RNA interference
Applications Tested/Suitable for HAMP sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for HAMP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_445921.1
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NCBI GenBank Nucleotide #
NM_053469.1
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UniProt Primary Accession #
Q99MH3
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UniProt Related Accession #
Q99MH3[Other Products]
Molecular Weight
9,286 Da
NCBI Official Full Name
hepcidin
NCBI Official Synonym Full Names
hepcidin antimicrobial peptide
NCBI Official Symbol
Hamp [Similar Products]
NCBI Official Synonym Symbols
Hepc
[Similar Products]
NCBI Protein Information
hepcidin
UniProt Protein Name
Hepcidin
UniProt Gene Name
Hamp [Similar Products]
UniProt Synonym Gene Names
Hepc [Similar Products]
UniProt Entry Name
HEPC_RAT
NCBI Summary for HAMP
may play a role in the regulation of iron metabolism [RGD, Feb 2006]
UniProt Comments for HAMP
HAMP: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B); also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy. Belongs to the hepcidin family.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; apical cortex; cell; cytoplasm; extracellular region; nucleus
Molecular Function: copper ion binding; hormone activity
Biological Process: iron ion homeostasis; cellular iron ion homeostasis; negative regulation of inflammatory response; myeloid cell homeostasis; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; negative regulation of bone resorption; JAK-STAT cascade; defense response to fungus; positive regulation of macrophage activation
Research Articles on HAMP
1. ferristatin II synergized with BMP6 and IL-6 to enhance hepcidin expression in vitro, However, this synergy was not due to activation of either Smad or Stat3 signaling
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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