Product Name
ADCY10, Polyclonal Antibody
Popular Item
Full Product Name
ADCY10 Antibody (Center)
Product Synonym Names
Adenylate cyclase type 10; AH-related protein; Adenylate cyclase homolog; Germ cell soluble adenylyl cyclase; hsAC; sAC; Testicular soluble adenylyl cyclase; ADCY10; SAC
Product Gene Name
anti-ADCY10 antibody
[Similar Products]
Antibody/Peptide Pairs
ADCY10 peptide (MBS9225820) is used for blocking the activity of ADCY10 antibody (MBS9207388)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
557-583
3D Structure
ModBase 3D Structure for Q96PN6
Specificity
This ADCY10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 557-583 amino acids from the Central region of human ADCY10.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.42 (lot specific)
Antigen Type
Synthetic Peptide
Function
Catalyzes the formation of the signaling molecule cAMP (PubMed:12609998, PubMed:15659711,PubMed:24616449, PubMed:25040695,PubMed:24567411). May function as sensor that mediates responses to changes in cellular bicarbonate and CO(2) levels (PubMed:15659711, PubMed:17591988). Has a critical role in mammalian spermatogenesis by producing the cAMP which regulates cAMP-responsive nuclear factors indispensable for sperm maturation in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization (By similarity). Involved inciliary beat regulation (PubMed:17591988).
Cellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region Nucleus. Cell projection, cilium. Cytoplasm. Mitochondrion. Note=Distributed to subcellular compartments containing cAMP targets. Found as a plasma membraneassociated protein, protein concentrated in the perinuclear region and protein colocalized with actin or tubulin
Tissue Location
Detected in airway epithelial cells and testis (at protein level) (PubMed:17591988). Weakly expressed in multiple tissues. Expressed in brain, heart, kidney, liver, lung, pancreas, peripheral blood leukocytes, placenta, skeletal muscle, stomach, thymus, airway epithelial cells, duodenum, jejunum and ileum. Very low level of expression in bone.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-ADCY10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-ADCY10 antibody
Cancer; Signal Transduction
Applications Tested/Suitable for anti-ADCY10 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes for anti-ADCY10 antibody
WB: 1:1000
FC: 1:10-50
Western Blot (WB) of anti-ADCY10 antibody
Anti-ADCY10 Antibody (Center) at 1:1000 dilution + K562 whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 187 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Flow Cytometry (FC/FACS) of anti-ADCY10 antibody
ADCY10 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NCBI/Uniprot data below describe general gene information for ADCY10. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001161221.1
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NCBI Related Accession #
HumanNP_060887.2[Other Products]
NCBI GenBank Nucleotide #
NM_001167749.2
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UniProt Primary Accession #
Q96PN6
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UniProt Secondary Accession #
O95558; Q5R329; Q5R330; Q8WXV4; Q9NNX0; B4DZF0; F5GWS5[Other Products]
UniProt Related Accession #
Q96PN6[Other Products]
NCBI Official Full Name
adenylate cyclase type 10 isoform 2
NCBI Official Synonym Full Names
adenylate cyclase 10 (soluble)
NCBI Official Symbol
ADCY10 [Similar Products]
NCBI Official Synonym Symbols
SAC; HCA2; SACI; Sacy; hsAC
[Similar Products]
NCBI Protein Information
adenylate cyclase type 10
UniProt Protein Name
Adenylate cyclase type 10
UniProt Synonym Protein Names
AH-related protein; Adenylate cyclase homolog; Germ cell soluble adenylyl cyclase; hsAC; sAC; Testicular soluble adenylyl cyclase
Protein Family
Adenylate cyclase
UniProt Gene Name
ADCY10 [Similar Products]
UniProt Synonym Gene Names
SAC; hsAC; sAC [Similar Products]
UniProt Entry Name
ADCYA_HUMAN
NCBI Summary for ADCY10
The protein encoded by this gene belongs to a distinct class of adenylyl cyclases that is soluble and insensitive to G protein or forskolin regulation. Activity of this protein is regulated by bicarbonate. Variation at this gene has been observed in patients with absorptive hypercalciuria. Alternatively spliced transcript variants encoding different isoforms have been observed. There is a pseudogene of this gene on chromosome 6. [provided by RefSeq, Jul 2014]
UniProt Comments for ADCY10
SAC: Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor. Involved in ciliary beat regulation. Genetic variations in ADCY10 are a cause of susceptibility to hypercalciuria absorptive type 2 (HCA2). Absorptive hypercalciuria is a common type of hypercalciuria, a condition characterized by excessive urinary calcium excretion. Absorptive hypercalciuria is due to gastrointestinal hyperabsorption of calcium and is a frequent cause of calcium oxalate nephrolithiasis. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 4.6.1.1; Lyase; Adenylyl cyclase; Nucleotide Metabolism - purine
Chromosomal Location of Human Ortholog: 1q24
Cellular Component: growth cone; cytoskeleton; cell soma; axon; apical part of cell; perinuclear region of cytoplasm; cytoplasm; dendrite; plasma membrane; cytosol
Molecular Function: manganese ion binding; magnesium ion binding; ATP binding; ATPase binding; adenylate cyclase activity
Biological Process: positive regulation of apoptosis; cAMP biosynthetic process; spermatogenesis
Disease: Hypercalciuria, Absorptive, 2
Research Articles on ADCY10
1. Evaluation of soluble adenylyl cyclase expression using R21 antibody is a useful diagnostic adjunct in the evaluation of margins of LM during slow Mohs surgery.
Precautions
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