Product Name
Patched (PTCH), Polyclonal Antibody
Full Product Name
Patched Antibody
Product Gene Name
anti-PTCH antibody
[Similar Products]
Product Synonym Gene Name
PTCH1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13635
Species Reactivity
Human, Mouse
Specificity
Patched Antibody detects endogenous levels of total Patched protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human Patched.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-PTCH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-PTCH antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-PTCH antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000
Immunohistochemistry (IHC) of anti-PTCH antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Patched Antibody. The picture on the right is blocked with the synthesized peptide.
Western Blot (WB) of anti-PTCH antibody
Western blot analysis of lysates from mouse muscle cells, using Patched Antibody. The lane on the right is blocked with the synthesized peptide.
NCBI/Uniprot data below describe general gene information for PTCH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000255.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000264.4
[Other Products]
UniProt Primary Accession #
Q13635
[Other Products]
UniProt Secondary Accession #
Q13463; Q5R1U7; Q5R1U9; Q5R1V0; Q5VZC0; Q5VZC2; Q86XG7; A3KBI9; E9PEJ8[Other Products]
UniProt Related Accession #
Q13635[Other Products]
NCBI Official Full Name
protein patched homolog 1 isoform L
NCBI Official Synonym Full Names
patched 1
NCBI Official Symbol
PTCH1 [Similar Products]
NCBI Official Synonym Symbols
PTC; BCNS; PTC1; PTCH; NBCCS
[Similar Products]
NCBI Protein Information
protein patched homolog 1
UniProt Protein Name
Protein patched homolog 1
Protein Family
Patched-related protein
UniProt Gene Name
PTCH1 [Similar Products]
UniProt Synonym Gene Names
PTCH; PTC; PTC1 [Similar Products]
NCBI Summary for PTCH
This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017]
UniProt Comments for PTCH
Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.
Research Articles on PTCH
1. The authors found 15 germline PTCH1 mutations, uniformly distributed across the PTCH1 gene among patients with Basaloid Follicular Hamartoma and Basal Cell Carcinoma. Therefore, Basaloid Follicular Hamartoma and Basal Cell Carcinoma may be the same genetic entity and not two distinctive syndromes.
Precautions
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Disclaimer
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