Dedicator of Cytokinesis Protein 8 (DOCK8), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of DOCK8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9394609 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dedicator of Cytokinesis Protein 8 (DOCK8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DOCK8. The ELISA analytical biochemical technique of the MBS9394609 kit is based on DOCK8 antibody-DOCK8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DOCK8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DOCK8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

227,477 Da

dedicator of cytokinesis 8

dedicator of cytokinesis protein 8

Dedicator of cytokinesis protein 8

This gene encodes a member of the DOCK180 family of guanine nucleotide exchange factors. Guanine nucleotide exchange factors interact with Rho GTPases and are components of intracellular signaling networks. Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jun 2010]

DOCK8: Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP. Defects in DOCK8 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal recessive (AR-HIES). It is a rare disorder of immunity characterized by immunodeficiency, recurrent infections, eczema, increased serum IgE, eosinophilia and lack of connective tissue and skeletal involvement. Defects in DOCK8 are the cause of mental retardation autosomal dominant type 2 (MRD2). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. A chromosomal aberration disrupting DOCK8 has been found in a patient with mental retardation and ectodermal dysplasia. DOCK8 is disrupted in patients with mental retardation. A balanced translocation, t(X;9) (q13.1;p24). Belongs to the DOCK family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9p24.3

Cellular Component: cytosol; membrane

Molecular Function: protein binding

Biological Process: blood coagulation

Disease: Hyper-ige Recurrent Infection Syndrome, Autosomal Recessive; Mental Retardation, Autosomal Dominant 2

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