CD35, Monoclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

E11

Mouse

Purified
Purified IgG - liquid

IgG concentration 1 mg/ml (lot specific)

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Small volumes of anti-CD35 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse anti Human CD35 antibody, clone E11 recognizes CD35, a single chain cell surface glycoprotein which exists in four allotypic forms (A,B,C,D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement. Clone E11 has been reported to be suitable for use in Western Blotting (Birmingham et al. 1996)

Immunohistology Frozen, Flow cytometry (FC/FACS), Immunohistology Paraffin*, Western Blot (WB)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Immunohistology - Frozen: Minimum Dilution: 1ug/ml; Maximum Dilution: 10ug/ml
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200
Immunohistology - Paraffin: Minimum Dilution: 1ug/ml; Maximum Dilution: 10ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Staining of human peripheral blood monocytes with Mouse anti Human CD35

Staining of human peripheral blood granulocytes with Mouse anti Human CD35:RPE (MBS213854)

Staining of human peripheral blood lymphocytes with Mouse anti Human CD35 (MBS213086)

223,663 Da

complement component (3b/4b) receptor 1 (Knops blood group)

complement receptor type 1; C3-binding protein; C3b/C4b receptor; CD35 antigen; Knops blood group antigen

Complement receptor type 1

C3BR  [Similar Products]

CR1_HUMAN

This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized. [provided by RefSeq, Jul 2008]

CR1: Mediates cellular binding of particles and immune complexes that have activated complement. Belongs to the receptors of complement activation (RCA) family.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: complement component C3b receptor activity; complement component C4b binding; complement component C4b receptor activity; complement component C3b binding

Biological Process: negative regulation of complement activation, alternative pathway; complement receptor mediated signaling pathway; negative regulation of complement activation, classical pathway; regulation of complement activation; innate immune response; complement activation, classical pathway

Disease: Knops Blood Group System; Malaria, Susceptibility To

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