CD35, C3b, Monoclonal Antibody

Anti -CD35, C3b (CR1, Follicular Dendritic Cell Marker)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 1; NC_000001.10 (207669473..207815110). Location: 1q32

Monoclonal

IgG1

9G147

Mouse

Recognizes a single epitope of CR1.

Affinity Purified
Purified

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-CR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CD35 (CR1) is a type 1 membrane glycoprotein participating in clearance of immune complexes, regulation of autologous complement activation on cell surfaces, and complement- dependent cellular cytotoxicity mediated by leukocytes. CD35 inhibits complement activation by two distinct mechanisms. CD35 is a cofactor for factor I cleavage of C3b, iC3b and C4b. Furthermore CD35 mediates decay-dissociation of C3 and C5 convertases. CD35 is found on erythrocytes, neutrophils, monocytes and dendritic cells. There are four allotypic variants of CR35 (CR1) with different molecular masses: 190kD (CR-1A), 220kD (CR1-B), 160kD (CR1-C) and 250kD (CR1-D).

Antibodies; Abs to CD Markers

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Dilution: Flow Cytometry: 1:10
Western Blot: 1:10
Immunohistochemistry: 1:10
Immunoprecipitation: 1:10

223,663 Da[Similar Products]

complement component (3b/4b) receptor 1 (Knops blood group)

complement receptor type 1; CD35 antigen; C3b/C4b receptor; C3-binding protein; Knops blood group antigen

Complement receptor type 1

C3BR  [Similar Products]

CR1_HUMAN

This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized. [provided by RefSeq, Jul 2008]

CR1: Mediates cellular binding of particles and immune complexes that have activated complement. Belongs to the receptors of complement activation (RCA) family.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: complement component C4b binding; complement component C3b receptor activity; complement component C4b receptor activity; complement component C3b binding

Biological Process: negative regulation of complement activation, alternative pathway; complement receptor mediated signaling pathway; negative regulation of complement activation, classical pathway; regulation of complement activation; innate immune response; complement activation, classical pathway

Disease: Knops Blood Group System; Malaria, Susceptibility To

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