EWSR1, Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Affinity Purified

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-EWSR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This is a rabbit polyclonal antibody against EWSR1. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: EWSR1 is a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products.This gene encodes a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products.

Polyclonal; RNA Binding Proteins; Transcription Regulation; Disease Related; DNA/RNA/Protein Interactions; Transcription Factors;

Immunohistochemistry (IHC), Western Blot (WB)

Rabbit Anti-EWSR1 antibody
Paraffin Embedded Tissue: Human Heart cell Cellular Data: cardiac cell of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Lanes:
1. 30 ug MiaPaca-2 cell lysate
2. 30 ug Panc-1 cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-Rabbit HRP
Secondary Antibody Dilution:
1:4000
Gene Name:
EWSR1
Submitted by:
Dr. Crissy Dudgeon, Ph.D., CINJ

Lanes:
Lane 1: 50ug Hela Lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:10,000
Gene Name:
EWSR1
Submitted by:
Archa Fox, University of Western Australia
EWSR1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells

Sample Type: HEK cells transfected with pFLAG EWSR1-ATF1Primary Dilution: 1:1000EWSR1 is supported by BioGPS gene expression data to be expressed in HEK293

WB Suggested Anti-EWSR1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate

68kDa

EWS RNA binding protein 1

RNA-binding protein EWS

RNA-binding protein EWS

EWS  [Similar Products]

EWS_HUMAN

This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009]

EWS: an oncogenic RNA-binding protein. Might normally function as a repressor. Binds RPB3, SF1, calmodulin and RNA. Interacts with and regulated by Pyk2. Relocates from cytoplasm to ribosomes upon Pyk2 activation. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. 5 alternatively-spliced isoforms have been described.

Protein type: Oncoprotein; Transcription, coactivator/corepressor; RNA-binding

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleolus; nucleus

Molecular Function: calmodulin binding; identical protein binding; protein binding; zinc ion binding; RNA binding; nucleotide binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent

Disease: Ewing Sarcoma

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