FIG4/SAC3, Monoclonal Antibody

Polyphosphoinositide phosphatase; 5-bisphosphate 5-phosphatase; Fig4; KIAA0274; Sac3; Phosphatidylinositol 3; Phosphatidylinositol 3;5 bisphosphate 5 phosphatase; SAC domain containing protein 3; SAC domain-containing protein 3; SAC3 antibody

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

S202-7

Mouse

Protein G Purified

1mg/mL (lot specific)

-20 degree C

Small volumes of anti-Fig4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background Info: Detects a 100 kDa protein.

Scientific Background: Fig4/Sac3 phosphatase, a 97-kDa protein, specifically removes the 5-phosphate from the inositol ring of PI(3,5)P2, a membrane-bound phospholipid, which acts as a molecular signal for trafficking and fusion of intracellular vesicles. It is broadly expressed in all eukaryotic cells and plays a vital role in intracellular membrane trafficking and signaling. Mutations in Fig4/Sac3 have been linked to pale tremor mice that exhibit neuropathy with severe central and peripheral neuronal degeneration, including the loss of neurons in the dorsal root ganglion and the sciatic nerve. Fig4/Sac3 knockout mice have been shown to be smaller in size and exhibit abnormal tremor and gait and neuronal autophagy.

Neuroscience

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

1:1000 WB

Western Blot analysis of Mouse Brain Membrane showing detection of ~100 kDa FIG4/SAC3 protein using Mouse Anti-FIG4/SAC3 Monoclonal Antibody, Clone S202-7. Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-FIG4/SAC3 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.

103,447 Da[Similar Products]

FIG4 homolog (S. cerevisiae)

polyphosphoinositide phosphatase; SAC domain-containing protein 3; Sac domain-containing inositol phosphatase 3; phosphatidylinositol 3,5-bisphosphate 5-phosphatase

Polyphosphoinositide phosphatase

Kiaa0274; Sac3  [Similar Products]

FIG4_MOUSE

SAC3: The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). In vitro, hydrolyzes all three D5-phosphorylated polyphosphoinositide substrates in the order PtdIns(4,5)P2 > PtdIns(3,5)P2 > PtdIns(3,4,5)P3. Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes. Defects in FIG4 are the cause of Charcot-Marie-Tooth disease type 4J (CMT4J). CMT4J is a recessive demyelinating, severe form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies characterized by severely reduced motor nerve conduction velocities (NCVs) (less than 38m/s) and segmental demyelination and remyelination, and primary peripheral axonal neuropathies characterized by normal or mildly reduced NCVs and chronic axonal degeneration and regeneration on nerve biopsy. Defects in FIG4 are the cause of amyotrophic lateral sclerosis type 11 (ALS11). ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.

Protein type: EC 3.1.3.-

Cellular Component: Golgi apparatus; recycling endosome; membrane; endoplasmic reticulum; endosome membrane; endosome

Molecular Function: protein tyrosine/threonine phosphatase activity; phosphatidylinositol-3-phosphatase activity; inositol-1,3,4-trisphosphate 4-phosphatase activity; hydrolase activity; phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity; inositol-1,4,5,6-tetrakisphosphate 6-phosphatase activity; inositol bisphosphate phosphatase activity; phosphoric ester hydrolase activity; inositol-1,3,4,5,6-pentakisphosphate 3-phosphatase activity; transmembrane receptor protein phosphatase activity; JUN kinase phosphatase activity; 5-amino-6-(5-phosphoribitylamino)uracil phosphatase activity; phosphatidylinositol-4-phosphate phosphatase activity; NADP phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; inositol-4,5-bisphosphate 5-phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity; phosphohistidine phosphatase activity

Biological Process: myelin formation; negative regulation of myelination; dephosphorylation; pigmentation; neuron development; locomotory behavior; phosphatidylinositol metabolic process; vacuole organization and biogenesis

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