GTPase HRas, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay recognizes recombinant and natural rat GTPase HRas. No significant cross-reactivity or interference was observed.

For long term storage, please store the entire kit at -20 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of Hras1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS288157 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GTPase HRas (Hras1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Hras1. The ELISA analytical biochemical technique of the MBS288157 kit is based on Hras1 antibody-Hras1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Hras1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Hras1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GTPase HRas. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GTPase HRas and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GTPase HRas, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GTPase HRas in the samples is then determined by comparing the O.D. of the samples to the standard curve.

21,298 Da

Harvey rat sarcoma virus oncogene

GTPase HRas

GTPase HRas

Hras1  [Similar Products]

RASH_RAT

mutated in rats exposed to the carcinogen 2-amino-1-methyl-6phenylimidazo[4;5-b]pyridine (PhiP); may be involved in the pathogenesis of chemically induced mammary tumors [RGD, Feb 2006]

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

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