Full Product Name
AML1 (Phospho-Ser435) Antibody
Product Synonym Names
Acute myeloid leukemia 1 protein; CBF-alpha 2; CBFA2; Core-binding factor; alpha 2 subunit; Oncogene AML-1; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer binding protein 2 alpha B subunit; RUN1; Runt-related transcription factor 1; RUNX1; SL3-3 enhan
Product Gene Name
anti-AML1 antibody
[Similar Products]
Product Synonym Gene Name
RUNX1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q01196
Species Reactivity
Human, Mouse, Rat
Specificity
AML1 (Phospho-Ser435) Antibody detects endogenous levels of RUNX1 only when phosphorylated at Ser435.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Phospho
Modification Sites
H:S435; M:S435; R:S434
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human AML1 around the phosphorylation site of Ser435.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-AML1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AML1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-AML1 antibody
WB: 1:500~1:1000
ELISA: 1:10000
ELISA (EIA) of anti-AML1 antibody
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using AML1 (Phospho-Ser435) Antibody
Western Blot (WB) of anti-AML1 antibody
Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using AML1 (Phospho-Ser435) Antibody.
NCBI/Uniprot data below describe general gene information for AML1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001001890.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001001890.2
[Other Products]
UniProt Primary Accession #
Q01196
[Other Products]
UniProt Secondary Accession #
O60472; O60473; O76047; O76089; Q13081; Q13755; Q13756; Q13757; A8MV94; B2RMS4; D3DSG1[Other Products]
UniProt Related Accession #
Q01196[Other Products]
NCBI Official Full Name
runt-related transcription factor 1 isoform AML1b
NCBI Official Synonym Full Names
runt related transcription factor 1
NCBI Official Symbol
RUNX1 [Similar Products]
NCBI Official Synonym Symbols
AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
[Similar Products]
NCBI Protein Information
runt-related transcription factor 1
UniProt Protein Name
Runt-related transcription factor 1
UniProt Synonym Protein Names
Acute myeloid leukemia 1 protein; Core-binding factor subunit alpha-2; CBF-alpha-2; Oncogene AML-1; Polyomavirus enhancer-binding protein 2 alpha B subunit; PEA2-alpha B; PEBP2-alpha B; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit
UniProt Gene Name
RUNX1 [Similar Products]
UniProt Synonym Gene Names
AML1; CBFA2; CBF-alpha-2; PEA2-alpha B; PEBP2-alpha B [Similar Products]
NCBI Summary for AML1
Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for AML1
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532). Positively regulates the expression of RORC in T-helper 17 cells ().
Research Articles on AML1
1. The here presented transcriptomic subgroup-based approach unified the gene expression profiles of RUNX1-CBFA2T3 and RUNX1-RUNX1T1 acute myeloid leukemia.
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