B7-H4, Cell Line

For Research Use Only. Not for use in diagnostic procedures.

Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

Immediately upon receipt, store in liquid nitrogen.

Small volumes of B7-H4 cell line vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

mB7-H4 Stable Cell Line is a stably transfected CHO-K1 cell line which expresses mouse B7-H4, also known as VTCN1.

Cell Lines; Stable Cell Lines

Flow Cytometry, Immunocytochemistry, Western blotting

Screen for antibodies of mouse B7-H4 through Flow Cytometry, Immunocytochemistry or Western blotting.
Culture conditions:
Cells should be grown at 37 degree C with 5% CO2 using DMEM medium supplemented with 10% FBS and 1% Pen/Strep, plus 10ug/ml of Blasticidin.
It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37 degree C water-bath, transfer to a tube containing 10 ml of growth medium without Blasticidin, spin down cells, resuspend cells in pre-warmed growth o medium without Blasticidin, transfer resuspended cells to T25 flask and culture in 37 degree C-CO2 incubator.
Leave the T25 flask in the incubator for 1~2 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Blasticidin. Cells should be split before they reach complete confluence.
To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly.

Fig-1: Detection of mouse B7-H4 in the CHO-K1/mB7-H4 stable cell line by Flow Cytometry. CHO-K1 cells (Green); CHO-K1/mB7-H4 cells (Red).

V-set domain containing T-cell activation inhibitor 1

V-set domain-containing T-cell activation inhibitor 1

V-set domain-containing T-cell activation inhibitor 1

B7-H4  [Similar Products]

This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

VTCN1: Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor- associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.1-p12

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