RAB7, Polyclonal Antibody

Ras-related protein Rab-7a; RAB7A; RAB7

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This RAB7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-204 amino acids from the C-terminal region of human RAB7.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.28 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-RAB7A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. RAB7 is a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori.

Signal Transduction

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

WB~~1:1000

Western blot analysis of RAB7 Antibody (C-term) in K562 cell line lysates (35ug/lane). RAB7 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with RAB7 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

RAB7 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

23490

RAB7A, member RAS oncogene family

ras-related protein Rab-7a

Ras-related protein Rab-7a

RAB7  [Similar Products]

RAB7A_HUMAN

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]

Rab7: a member of the RAB family of RAS-related GTP-binding proteins, important regulators of vesicular transport and are located in specific intracellular compartments. RAB7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori. Mutations in this protein cause Charcot-Marie-Tooth type 2B neuropathy.

Protein type: G protein, monomeric; G protein, monomeric, Rab; G protein

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: Golgi apparatus; phagocytic vesicle membrane; intracellular membrane-bound organelle; lysosome; lysosomal membrane; retromer complex; late endosome; vacuolar membrane; pre-autophagosomal structure membrane; melanosome; phagocytic vesicle

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; Rac GTPase binding

Biological Process: protein transport; protein targeting to lysosome; early endosome to late endosome transport; antigen processing and presentation of exogenous peptide antigen via MHC class II; endosome to lysosome transport; endocytosis; protein to membrane docking; Rab protein signal transduction; retrograde transport, endosome to Golgi; epidermal growth factor ligand processing; bone resorption

Disease: Charcot-marie-tooth Disease, Axonal, Type 2b

Davies,J.P., et.al., Genomics 41 (1), 131-134 (1997)
Vitelli,R., et.al., Biochem. Biophys. Res. Commun. 229 (3), 887-890 (1996)

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