MYBPC3, Polyclonal Antibody

Myosin-binding protein C; cardiac-type; Cardiac MyBP-C; C-protein; cardiac muscle isoform; MYBPC3

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit IgG

Rabbit

This MYBPC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the N-terminal region of human MYBPC3.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-MYBPC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3, the cardiac isoform, is expressed exclussively in heart muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase (PKA) upon adrenergic stimulation may be linked to modulation of cardiac contraction. Mutations in MYBPC3 are one cause of familial hypertrophic cardiomyopathy.

Cardiovascular

ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

IHC-P~~1:25
WB~~1:1000

MBS9215190 staining MYBPC3 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

MBS9215190staining MYBPC3 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

MBS9215190 staining MYBPC3 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes : Anti-MYBPC3 Antibody (N-term) at 1:2000 dilution Lane 1: mouse heart lysate Lane 2: rat heart lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 141 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-MYBPC3 Antibody (N-term) at 1:1000-1:2000 dilution Lane 1: human liver lysate Lane 2: mouse heart lysate Lane 3: rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 141 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysate from rat heart tissue lysate, using MYBPC3 Antibody (N-term). MBS9215190 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot analysis of lysate from mouse heart tissue lysate, using MYBPC3 Antibody (N-term). MBS9215190 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

140762

myosin binding protein C, cardiac

myosin-binding protein C, cardiac-type

Myosin-binding protein C, cardiac-type

Cardiac MyBP-C  [Similar Products]

MYPC3_HUMAN

MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3, the cardiac isoform, is expressed exclussively in heart muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase (PKA) upon adrenergic stimulation may be linked to modulation of cardiac contraction. Mutations in MYBPC3 are one cause of familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

MYBPC3: Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Belongs to the immunoglobulin superfamily. MyBP family.

Protein type: Myosin-binding

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: sarcomere; striated muscle thick filament; cytosol; A band

Molecular Function: identical protein binding; ATPase activator activity; myosin binding; structural constituent of muscle; metal ion binding; titin binding; actin binding; myosin heavy chain binding

Biological Process: heart morphogenesis; regulation of heart rate; positive regulation of ATPase activity; regulation of muscle filament sliding; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; sarcomere organization; myosin filament assembly; cell adhesion; cardiac muscle contraction; muscle filament sliding

Disease: Left Ventricular Noncompaction 10; Cardiomyopathy, Familial Hypertrophic, 4

Millat, G., et al. Clin. Chim. Acta 411 (23-24), 1983-1991 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Millat, G., et al. Eur J Med Genet 53(5):261-267(2010)
Zimmerman, R.S., et al. Genet. Med. 12(5):268-278(2010)
Brion, M., et al. Ann. Clin. Lab. Sci. 40(3):285-289(2010)

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.