SAMHD1, Monoclonal Antibody

Mouse Monoclonal [clone 3F5] (IgG2b) to Human SAMHD1; Human SAMHD1; AGS5; CHBL2; DCIP; HDDC1; Monocyte protein 5; MOP-5; MOP5; SBBI88; Mg11; SAM domain and HD domain 1

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2b

3F5

Mouse

Human SAMHD1 / DCIP

Protein A/G purified

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

1 mg/ml (lot specific)

Store at -20 degree C. Minimize freezing and thawing.

Small volumes of anti-SAMHD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

SAMHD1 Antibody, AGS5 Antibody, CHBL2 Antibody, DCIP Antibody, HDDC1 Antibody, Monocyte protein 5 Antibody, MOP-5 Antibody, MOP5 Antibody, SBBI88 Antibody, Mg11 Antibody, SAM domain and HD domain 1 Antibody Description: Host restriction nuclease that blocks early-stage virus replication in dendritic and other myeloid cells. Likewise, suppresses LINE-1 retrotransposon activity. May function by reducing the cellular dNTP levels to levels too low for retroviral reverse transcription to occur. May play a role in mediating proinflammatory responses to TNF-alpha signaling.

Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)

Flow Cytometry (1:100)
IF (1:100)
IHC-P (5 ug/ml)
WB (1:200 - 1:500)

Anti-SAMHD1 antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-SAMHD1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.

Western blot of extracts (35 ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.

Flow cytometry of HeLa cells, using anti-SAMHD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow cytometry of Jurkat cells, using anti-SAMHD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

68,122 Da

SAM domain and HD domain 1

deoxynucleoside triphosphate triphosphohydrolase SAMHD1

Deoxynucleoside triphosphate triphosphohydrolase SAMHD1

MOP5; dNTPase; DCIP; MOP-5  [Similar Products]

SAMH1_HUMAN

This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome. [provided by RefSeq, Mar 2010]

SAMHD1: a protein induced by interferon gamma.

Protein type: EC 3.1.4.-

Chromosomal Location of Human Ortholog: 20pter-q12

Cellular Component: intracellular; nucleoplasm; nucleus; plasma membrane

Molecular Function: dGTP binding; dGTPase activity; nucleic acid binding; protein binding; RNA binding; zinc ion binding

Biological Process: cytokine and chemokine mediated signaling pathway; dATP catabolic process; defense response to virus; dGTP catabolic process; immune response; protein homotetramerization; regulation of innate immune response

Disease: Aicardi-goutieres Syndrome 5; Chilblain Lupus 2

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