RAB7A, Polyclonal Antibody

PRO2706; RAB7

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity Purification

Store at 4 degree C. Avoid freeze/thaw cycles.

Small volumes of anti-RAB7A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

Primary antibody

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

WB: 1:500 - 1:2000
IHC: 1:50- 1:100
FC: 1:20- 1:50

Western blot analysis of extracts of various cell lines, using RAB7A antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

23kDa

RAB7A, member RAS oncogene family

ras-related protein Rab-7a

Ras-related protein Rab-7a

RAB7  [Similar Products]

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]

Rab7: a member of the RAB family of RAS-related GTP-binding proteins, important regulators of vesicular transport and are located in specific intracellular compartments. RAB7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori. Mutations in this protein cause Charcot-Marie-Tooth type 2B neuropathy.

Protein type: G protein; G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: cytosol; endosome membrane; late endosome; late endosome membrane; lipid particle; lysosomal membrane; lysosome; phagocytic vesicle; plasma membrane; retromer complex; secretory granule membrane

Molecular Function: GDP binding; GTP binding; GTPase activity; protein binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; autophagic vacuole formation; early endosome to late endosome transport; endocytosis; endosome to lysosome transport; epidermal growth factor ligand processing; neutrophil degranulation; positive regulation of protein catabolic process; positive regulation of viral reproduction; protein targeting to lysosome; protein to membrane docking; protein transport; release of virus from host; retrograde transport, endosome to Golgi

Disease: Charcot-marie-tooth Disease, Axonal, Type 2b

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