CD18, Monoclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

MEM-148

Mouse

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised

Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.

Small volumes of anti-CD18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse anti Human CD18 (Activation Epitope) antibody, clone MEM-148 recognizes an epitope on the human CD18 molecule that is hidden in the CD11/CD18 heterodimer on resting cells. Clone MEM-148 binds very weakly to resting peripheral blood leukocytes and strongly to all leukocytes upon cellular activation. The epitope recognized by Mouse anti Human CD18 (Activation Epitope) antibody, clone MEM-148 is also exposed during dissociation of the CD11/CD18 by low pH.

Flow cytometry (FC/FACS)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat

Staining of human peripheral blood monocytes with Mouse anti Human CD18 (Activation Epitope):Biotin (MBS210392)

Staining of human peripheral blood monocytes with Mouse anti Human CD86 (Activation Epitope): Azide free (MBS210203)

Staining of human peripheral blood granulocytes with Mouse anti Human CD18 (ACT EPITOPE) (MBS212479)

Staining of human peripheral blood granulocytes with Mouse anti Human CD18 (Activation Epitope):RPE (MBS213718)

84,782 Da

integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)

integrin beta-2; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); complement receptor C3 beta-subunit; integrin beta chain, beta 2; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C

Integrin beta-2

CD18; MFI7  [Similar Products]

ITB2_HUMAN

This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

ITGB2: the integrin beta 2 subunit. Can combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Participates in cell adhesion as well as cell-surface mediated signaling. Defects are the cause of leukocyte adhesion deficiency type I (LAD1).

Protein type: Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.; Cell adhesion

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cell surface; membrane; plasma membrane; integrin complex; vesicle; receptor complex; lipid raft

Molecular Function: protein binding; protein heterodimerization activity; metal ion binding; protein complex binding; ICAM-3 receptor activity; cell adhesion molecule binding; glycoprotein binding; protein kinase binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; apoptosis; cell-matrix adhesion; natural killer cell activation; receptor clustering; activation of NF-kappaB transcription factor; regulation of cell shape; leukocyte adhesion; cell-cell signaling; cellular extravasation; inflammatory response; cell adhesion; toll-like receptor 4 signaling pathway; regulation of peptidyl-tyrosine phosphorylation; aging; integrin-mediated signaling pathway; neutrophil chemotaxis; regulation of immune response; activated T cell proliferation; leukocyte migration during inflammatory response; heterotypic cell-cell adhesion; positive regulation of angiogenesis; toll-like receptor signaling pathway; endothelial cell migration; innate immune response; blood coagulation; leukocyte migration

Disease: Leukocyte Adhesion Deficiency, Type I

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