CD18 / IntegrinBeta-2 (ITGB2), Monoclonal Antibody

Beta 2 (antigen CD18 (p95); CD18; Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta; Cell surface adhesion glycoproteins LFA-1/CR3/p150; Complement component 3 receptor 3 and 4 subunit; Complement receptor C3 beta subunit; Integrin beta-2 (ITGB2); LAD; LCAMB; Leukocyte Cell Adhesion Molecule CD18; Lymphocyte function associated antigen 1 (LFA1); MAC1; Macrophage antigen 1 (mac1) beta

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a, kappa

68-5A5

Mouse

It recognizes a transmembrane glycoprotein of 95kDa, identified as CD18 or integrin -2 (Workshop III). It complexes non-covalently with either L, M, or X integrin (CD11a, b, or c) to form the heterodimers, LFA-1, Mac-1, and p150,95, respectively. LFA-1 is the receptor for three members of the Ig supergene family of proteins, ICAM-1 (CD54), ICAM-2 (CD102), and Mac-1 and p150,95 bind to ICAM-1, fibrinogen, and iC3b. ICAM-3 (CD50). CD18/CD11 heterodimeric molecules are involved with cell/cell and cell/extracellular adhesion in immune and inflammatory responses. This MAb blocks these cellular interactions.

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Small volumes of anti-ITGB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Functional Studies (Order Ab without Azide)
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)

95kDa

integrin subunit beta 2

integrin beta-2

Integrin beta-2

CD18; MFI7  [Similar Products]

ITB2_HUMAN

This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

ITGB2: the integrin beta 2 subunit. Can combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Participates in cell adhesion as well as cell-surface mediated signaling. Defects are the cause of leukocyte adhesion deficiency type I (LAD1).

Protein type: Cell adhesion; Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cell surface; membrane; plasma membrane; receptor complex

Molecular Function: cell adhesion molecule binding; glycoprotein binding; ICAM-3 receptor activity; protein binding; protein kinase binding

Biological Process: cell adhesion; cell-matrix adhesion; extracellular matrix organization and biogenesis; heterotypic cell-cell adhesion; leukocyte adhesion; leukocyte migration; neutrophil chemotaxis; receptor clustering; receptor internalization; regulation of immune response; regulation of peptidyl-tyrosine phosphorylation; toll-like receptor 4 signaling pathway

Disease: Leukocyte Adhesion Deficiency, Type I

Leucocyte Typing III, (ed. McMichael AJ et. al.), Oxford Univ. Press, Oxford, 1987

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