Product Name
NR2E3, siRNA
Full Product Name
NR2E3 siRNA (Human)
Product Synonym Names
PNR; RNR; Photoreceptor-specific nuclear receptor; Nuclear receptor subfamily 2 group E member 3; Retina-specific nuclear receptor
Product Gene Name
NR2E3 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9Y5X4
Specificity
NR2E3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NR2E3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of NR2E3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
NR2E3 sirna
siRNA to inhibit NR2E3 expression using RNA interference
Applications Tested/Suitable for NR2E3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for NR2E3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_055064.1
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NCBI GenBank Nucleotide #
NM_014249.3
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UniProt Primary Accession #
Q9Y5X4
[Other Products]
UniProt Secondary Accession #
Q9UHM4; B6ZGU0[Other Products]
UniProt Related Accession #
Q9Y5X4[Other Products]
Molecular Weight
39,638 Da
NCBI Official Full Name
photoreceptor-specific nuclear receptor isoform b
NCBI Official Synonym Full Names
nuclear receptor subfamily 2, group E, member 3
NCBI Official Symbol
NR2E3 [Similar Products]
NCBI Official Synonym Symbols
PNR; RNR; rd7; ESCS; RP37
[Similar Products]
NCBI Protein Information
photoreceptor-specific nuclear receptor
UniProt Protein Name
Photoreceptor-specific nuclear receptor
UniProt Synonym Protein Names
Nuclear receptor subfamily 2 group E member 3; Retina-specific nuclear receptor
Protein Family
Photoreceptor-specific nuclear receptor
UniProt Gene Name
NR2E3 [Similar Products]
UniProt Synonym Gene Names
PNR; RNR [Similar Products]
UniProt Entry Name
NR2E3_HUMAN
NCBI Summary for NR2E3
This protein is part of a large family of nuclear receptor transcription factors involved in signaling pathways. Nuclear receptors have been shown to regulate pathways involved in embryonic development, as well as in maintenance of proper cell function in *****s. Members of this family are characterized by discrete domains that function in DNA and ligand binding. This gene encodes a retinal nuclear receptor that is a ligand-dependent transcription factor. Defects in this gene are a cause of enhanced S cone syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Comments for NR2E3
NR2E3: Orphan nuclear receptor of retinal photoreceptor cells. Transcriptional factor that is an activator of rod development and repressor of cone development. Binds the promoter region of a number of rod- and cone-specific genes, including rhodopsin, M- and S-opsin and rod-specific phosphodiesterase beta subunit. Enhances rhodopsin expression. Represses M- and S-cone opsin expression. Defects in NR2E3 are a cause of enhanced S cone syndrome (ESCS). ESCS is an autosomal recessive retinopathy in which patients have increased sensitivity to blue light; perception of blue light is mediated by what is normally the least populous cone photoreceptor subtype, the S (short wavelength, blue) cones. ESCS is also associated with visual loss, with night blindness occurring from early in life, varying degrees of L (long, red)- and M (middle, green)-cone vision, and retinal degeneration. Defects in NR2E3 are the cause of retinitis pigmentosa type 37 (RP37). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP37 inheritance is autosomal dominant. Belongs to the nuclear hormone receptor family. NR2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor
Chromosomal Location of Human Ortholog: 15q23
Cellular Component: nucleoplasm; transcription factor complex; nucleus
Molecular Function: protein binding; ligand-dependent nuclear receptor activity; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity
Biological Process: transcription from RNA polymerase II promoter; negative regulation of cell proliferation; transcription initiation from RNA polymerase II promoter; eye photoreceptor cell development; intracellular receptor-mediated signaling pathway; visual perception; positive regulation of rhodopsin gene expression; retina development in camera-type eye; phototransduction; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; gene expression; negative regulation of transcription from RNA polymerase II promoter; signal transduction
Disease: Retinitis Pigmentosa 37; Enhanced S-cone Syndrome
Research Articles on NR2E3
1. Study presents evidence that PNR could promote ERalpha-negative breast cancer metastasis through activation of IL-13Ralpha2-mediated signaling pathway.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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