Product Name
4E-BP1, Polyclonal Antibody
Full Product Name
4E-BP1(Phospho-Thr36) Antibody
Product Synonym Names
BP-1; 4EBP1; 4E-BP1; PHAS-I
Product Gene Name
anti-4E-BP1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13541
Species Reactivity
Human, Mouse, Rat
Specificity
The antibody detects endogenous level of 4E-BP1 only when phosphorylated at threonine 36.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (lot specific)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of threonine 36(S-T-T(p)-P-G) derived from Human 4E-BP1.
Modification
Phospho-Thr36
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-4E-BP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-4E-BP1 antibody
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Product Categories/Family for anti-4E-BP1 antibody
Phospho-specific Ab
Applications Tested/Suitable for anti-4E-BP1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Western Blot (WB) of anti-4E-BP1 antibody
Western blot analysis of extracts from MDA-MB-435 cells, untreated or EGF-treated (200 ng/ml, 30min), using 4E-BP1 (Ab-36) antibody (#21215, Lane 1 and 2) and 4E-BP1 (phospho-Thr36) antibody (, Lane 3 and 4).
Immunohistochemistry (IHC) of anti-4E-BP1 antibody
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (phospho-Thr36) antibody ().
NCBI/Uniprot data below describe general gene information for 4E-BP1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_004086.1
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NCBI GenBank Nucleotide #
NM_004095.3
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UniProt Primary Accession #
Q13541
[Other Products]
UniProt Secondary Accession #
Q6IBN3; B2R502; D3DSW8[Other Products]
UniProt Related Accession #
Q13541[Other Products]
Molecular Weight
12,580 Da
NCBI Official Full Name
eukaryotic translation initiation factor 4E-binding protein 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol
EIF4EBP1 [Similar Products]
NCBI Official Synonym Symbols
BP-1; 4EBP1; 4E-BP1; PHAS-I
[Similar Products]
NCBI Protein Information
eukaryotic translation initiation factor 4E-binding protein 1
UniProt Protein Name
Eukaryotic translation initiation factor 4E-binding protein 1
UniProt Synonym Protein Names
Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
UniProt Gene Name
EIF4EBP1 [Similar Products]
UniProt Synonym Gene Names
4E-BP1; eIF4E-binding protein 1; PHAS-I [Similar Products]
UniProt Entry Name
4EBP1_HUMAN
NCBI Summary for 4E-BP1
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]
UniProt Comments for 4E-BP1
4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway.
Protein type: Translation initiation; Translation
Chromosomal Location of Human Ortholog: 8p12
Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol
Molecular Function: protein binding; translation repressor activity; eukaryotic initiation factor 4E binding
Biological Process: negative regulation of translational initiation; response to ethanol; TOR signaling pathway; cellular protein metabolic process; translation; translational initiation; insulin receptor signaling pathway; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; lung development; G1/S transition of mitotic cell cycle
Research Articles on 4E-BP1
1. ShcA drives breast tumor growth and angiogenesis in vivo in a 4E-BP-dependent manner
Precautions
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Disclaimer
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