4E-BP1, Polyclonal Antibody

4EBP1; EIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Insulin-stimulated EIF-4E binding protein PHAS-I; P/OKCL.6; PHAS-1; PHAS-I

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

4E-BP1 Antibody detects endogenous levels of total 4E-BP1 protein.

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1 mg/ml (lot specific)

Stable at -20 degree C for at least 1 year.

Small volumes of anti-4E-BP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:1000

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using 4E-BP1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from MDA-MB-435 cells, treated with EGF 200ng/ml 30', using 4E-BP1 Antibody. The lane on the right is blocked with the synthesized peptide.

12 kDa

eukaryotic translation initiation factor 4E binding protein 1

eukaryotic translation initiation factor 4E-binding protein 1

Eukaryotic translation initiation factor 4E-binding protein 1

4E-BP1; eIF4E-binding protein 1; PHAS-I  [Similar Products]

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

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