Laminin, ELISA Kit

Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2; laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2(laminin S); Lamb2; LAMB2_HUMAN; LAMC1; LAMC2; LAMC3; laminin A; Laminin B; Laminin B1s chain; Laminin gamma 1; Laminin gamma 2; Laminin gamma 3; Laminin subunit beta-2; Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; Laminin-9 subunit beta; OCCM; S-LAM beta; S-laminin subunit beta

For Research Use Only. Not for use in diagnostic procedures.

Natural rat Laminin

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of LAMA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS175992 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Laminin (LAMA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LAMA1. The ELISA analytical biochemical technique of the MBS175992 kit is based on LAMA1 antibody-LAMA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LAMA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LAMA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: rat Laminin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Laminin has been precoated onto 96-well plates. Standards(from rat sarcoma basement membrane) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Laminin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Laminin amount of sample captured in plate.
Background: Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation.2 The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids.1 The human laminin A chain is at locus 18p11.3.3 The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31.4 The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology.5 The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.6

Rat Laminin PicoKine ELISA Kit standard curve

196,474 Da

laminin, beta 2

laminin subunit beta-2; S-LAM beta; laminin chain B3; laminin chain beta 2; S-laminin subunit beta; laminin-3 subunit beta; laminin-4 subunit beta; laminin-7 subunit beta; laminin-9 subunit beta; laminin-11 subunit beta; laminin-14 subunit beta; laminin-15 subunit beta

Laminin subunit beta-2

S-LAM beta  [Similar Products]

LAMB2_RAT

a component of the basal lamina; may be involved in motor neuron function [RGD, Feb 2006]

LAMB2: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Defects in LAMB2 are the cause of Pierson syndrome (PIERSS); also known as microcoria-congenital nephrotic syndrome. Pierson syndrome is characterized by nephrotic syndrome with neonatal onset, diffuse mesangial sclerosis and eye abnormalities with microcoria as the leading clinical feature. Death usually occurs within the first weeks of life. Disease severity depends on the mutation type: nontruncating LAMB2 mutations may display variable phenotypes ranging from a milder variant of Pierson syndrome to isolated congenital nephrotic syndrome. Defects in LAMB2 are the cause of nephrotic syndrome type 5 with or without ocular abnormalities (NPHS5). NPHS5 is a renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. NPHS5 is characterized by very early onset of progressive renal failure. A subset of patients may develop mild ocular anomalies, such as myopia, nystagmus, and strabismus.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Cellular Component: extracellular matrix; laminin-3 complex; basement membrane; basal lamina; synapse

Molecular Function: integrin binding

Biological Process: cellular morphogenesis during differentiation; axon guidance; Schwann cell development; visual perception; axon extension involved in regeneration; retina development in camera-type eye; synapse organization and biogenesis; cell adhesion; astrocyte development; neurite development; neuromuscular junction development

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