Mediator of RNA polymerase II transcription subunit 12 (MED12), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of MED12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7211308 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MED12. The ELISA analytical biochemical technique of the MBS7211308 kit is based on MED12 antibody-MED12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MED12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MED12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Epigenetics and Nuclear Signaling

242,953 Da

mediator complex subunit 12

mediator of RNA polymerase II transcription subunit 12; CAG repeat protein 45; human opposite paired; OPA-containing protein; putative mediator subunit 12; activator-recruited cofactor 240 kDa component; trinucleotide repeat-containing gene 11 protein; thyroid hormone receptor-associated protein, 230 kDa subunit; mediator of RNA polymerase II transcription, subunit 12 homolog; thyroid hormone receptor-associated protein complex 230 kDa component; trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit)

Mediator of RNA polymerase II transcription subunit 12

ARC240; CAGH45; HOPA; KIAA0192; TNRC11; TRAP230; ARC240; Trap230  [Similar Products]

MED12_HUMAN

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009]

MED12: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3. Ubiquitous. Belongs to the Mediator complex subunit 12 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: nucleoplasm; centrosome; membrane; nucleolus; Srb-mediator complex; nucleus

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription coactivator activity; beta-catenin binding; transcription cofactor activity; thyroid hormone receptor binding; chromatin binding; receptor activity

Biological Process: steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Schwann cell development; negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway; positive regulation of transcription, DNA-dependent; heart development; stem cell maintenance; Wnt receptor signaling pathway through beta-catenin; androgen receptor signaling pathway; neural tube closure; gene expression; positive regulation of transcription from RNA polymerase II promoter; oligodendrocyte development

Disease: Ohdo Syndrome, X-linked; Opitz-kaveggia Syndrome; Lujan-fryns Syndrome

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