AML1, ELISA Kit

Acute myeloid leukemia 1 protein; CBF-alpha 2; CBFA2; Core-binding factor alpha 2 subunit; Oncogene AML-1; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer binding protein 2 alpha B subunit; RUN1; Runt-related transcription factor 1; RUNX1; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit

For Research Use Only. Not for use in diagnostic procedures.

Two 96-Well Plates

Store at 4 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of AML1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9501052 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AML1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AML1. The ELISA analytical biochemical technique of the MBS9501052 kit is based on AML1 antibody-AML1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AML1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AML1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Cell Based ELISA Kit

48797

runt related transcription factor 1

runt-related transcription factor 1

Runt-related transcription factor 1

AML1; CBFA2; CBF-alpha-2; PEA2-alpha B; PEBP2-alpha B  [Similar Products]

Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

AML1: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B- dependent transcriptional activation. Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Isoform AML-1L can neither bind DNA nor heterodimerize. Interacts with TLE1 and ALYREF/THOC4. Interacts with ELF1, ELF2 and SPI1. Interacts via its Runt domain with the ELF4 N-terminal region. Interaction with ELF2 isoform 2 (NERF-1a) may act to repress RUNX1-mediated transactivation. Interacts with KAT6A and KAT6B. Interacts with SUV39H1, leading to abrogation of transactivating and DNA-binding properties of RUNX1. Interacts with YAP1. Interacts with HIPK2. Interaction with CDK6 prevents myeloid differentiation, reducing its transcription transactivation activity. Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 21q22.12

Cellular Component: cytosol; intracellular membrane-bound organelle; nucleoplasm; nucleus

Molecular Function: ATP binding; protein binding; transcription regulatory region DNA binding

Biological Process: chondrocyte differentiation; hematopoietic stem cell proliferation; hemopoiesis; ossification; peripheral nervous system neuron development; positive regulation of angiogenesis; positive regulation of granulocyte differentiation; positive regulation of interleukin-2 production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of B cell receptor signaling pathway; regulation of bicellular tight junction assembly; regulation of cytokine-mediated signaling pathway; regulation of hematopoietic stem cell differentiation; regulation of intracellular estrogen receptor signaling pathway; regulation of keratinocyte differentiation; regulation of megakaryocyte differentiation; regulation of myeloid cell differentiation; regulation of regulatory T cell differentiation; regulation of Wnt receptor signaling pathway; transcription from RNA polymerase II promoter

Disease: Leukemia, Acute Myeloid; Platelet Disorder, Familial, With Associated Myeloid Malignancy; Rheumatoid Arthritis

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