BUB1B, Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures.

Sf9 insect cells using baculovirus

50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Small volumes of BUB1B recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: Recombinant human BUB1B (613-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: BUB1B or budding uninhibited by benzimidazoles 1 homolog beta is involved in spindle checkpoint function and has been localized to the kinetochore. BUB1B plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation (1). BUB1B links the regulation of chromosome-spindle attachment to mitotic checkpoint signaling (2). BUB1B expression during the spindle checkpoint is dependent on residual BUB1B expression and impaired spindle checkpoint function has been found in many forms of cancer. BUB1B is essential for early embryonic development and normal hematopoiesis.

Enzymes; Active Kinases

Kinase Assay, Western Blot (WB)

~73 kDa

BUB1 mitotic checkpoint serine/threonine kinase B

mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta

BUBR1; MAD3L; SSK1; hBUBR1  [Similar Products]

This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008]

BUB1B: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with CASC5. Induced during mitosis. Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. Kinase activity stimulated by CENPE. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: BUB family; EC 2.7.11.1; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: anaphase-promoting complex; cytoplasm; cytosol; kinetochore; outer kinetochore of condensed chromosome; perinuclear region of cytoplasm

Molecular Function: protein binding; protein kinase activity

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell proliferation; meiotic sister chromatid cohesion, centromeric; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; sister chromatid cohesion

Disease: Colorectal Cancer; Mosaic Variegated Aneuploidy Syndrome 1; Premature Chromatid Separation Trait

1. Rio Frio. Et.al: Homozygous BUB1B mutation and susceptibility to gastrointestinal neoplasia. New Eng. J. Med. 363: 2628-2637, 2010. 2. Lampson, M. A., Kapoor. et.al: The human mitotic checkpoint protein BubR1 regulates chromosome-spindle attachments. Nature Cell Biol. 7: 93-98, 2005.

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