Product Name
Midline-1 (Mid1), Recombinant Protein
Full Product Name
Recombinant Mouse Midline-1 (Mid1)
Product Gene Name
Mid1 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-680aa, Full length protein
Sequence
METLESELTC PICLELFEDP LLLPCAHSLC FNCAHRILVS HCATNEPVES INAFQCPTCR HVITLSQRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSET RRERAFDANT MSSAEKVLCQ FCDQDPAQDA VKTCVTCEVS YCDECLKATH PNKKPFTGHR LIEPIPDSHI RGLMCLEHED EKVNMYCVTD DQLICALCKL VGRHRDHQVA ALSERYDKLK QNLESNLTNL IKRNTELETL LAKLIQTCQH VEVNASRQEA KLTEECDLLI EIIQQRRQII GTKIKEGKVI RLRKLAQQIA NCKQCLERSA SLISQAEHSL KENDHARFLQ TAKNITERVS MATASSQVLI PEINLNDTFD TFALDFSREK KLLECLDYLT APNPPAIREE LCTASYDTIT VHWTSEDEFS VVSYELQYTI FTGQANVVNV ACDGTCLLGS AGLCNSADSW MIVPNIKQNH YTVHGLQSGT KYIFTVKAIN QAGSRSSEPG KLKTNSQPFR LDPKSAHRKL KVSHDNLTVE RDESSSKKSH APERFAGQGS YGVAGNVFID SGRHYWEVVT SGSTWYAIGL AYRSAPKHEW IGKNAASWAL CRCHNHWAVR HDGKETPIAP APHLRRVGVL LDYDNGSIAF YDALSSVHLH TFHAALAQPV CPTFTVWNKC LTIVTGLPIP DHLDCTEQRP
3D Structure
ModBase 3D Structure for O70583
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Mid1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Mid1 recombinant protein
This protein is a member of the tripartite motif (TRIM) family, also known as the RING-B box-coiled coil (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Several different transcript variants are generated by alternate splicing; however, the full-length nature of some of the variants has not been determined.
NCBI/Uniprot data below describe general gene information for Mid1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001277433.1
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NCBI GenBank Nucleotide #
NM_001290504.1
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UniProt Primary Accession #
O70583
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UniProt Secondary Accession #
O35418; Q7TPT6; B1AV00[Other Products]
UniProt Related Accession #
O70583[Other Products]
Molecular Weight
70,572 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase Midline-1 isoform 2
NCBI Official Synonym Full Names
midline 1
NCBI Official Symbol
Mid1 [Similar Products]
NCBI Official Synonym Symbols
Fxy; 61B3-R; Trim18; DXHXS1141
[Similar Products]
NCBI Protein Information
E3 ubiquitin-protein ligase Midline-1
UniProt Protein Name
E3 ubiquitin-protein ligase Midline-1
UniProt Synonym Protein Names
Midin; RING finger protein Midline-1; RING-type E3 ubiquitin transferase Midline-1Curated; Tripartite motif-containing protein 18
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
Mid1 [Similar Products]
UniProt Synonym Gene Names
Fxy; Trim18 [Similar Products]
UniProt Comments for Mid1
Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.
Research Articles on Mid1
1. we show that MID1 controls exocytosis of lytic granules and cytotoxicity in murine cytotoxic lymphocytes
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
