Full Product Name
Merlin (Phospho-Ser518) Antibody
Product Gene Name
anti-MERL antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P35240
Species Reactivity
Human, Mouse, Rat
Specificity
Merlin (phospho-Ser518) antibody detects endogenous levels of Merlin only when phosphorylated at serine 518.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Merlin around the phosphorylation site of serine 518 (R-L-SP-M-E).
Preparation and Storage
Store at-20 degree C for 1 year
Other Notes
Small volumes of anti-MERL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-MERL antibody
Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
Applications Tested/Suitable for anti-MERL antibody
Western Blot (WB)
Application Notes for anti-MERL antibody
WB: 1:500-1:1000
Western Blot (WB) of anti-MERL antibody
Western blot analysis of extracts from HUVEC cells untreated or treated with IFN-alpha (100ng/ml, 15min), using Merlin (Ab-518) antibody and Merlin (phospho-Ser518) antibody.
NCBI/Uniprot data below describe general gene information for MERL. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000259.1
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NCBI GenBank Nucleotide #
NM_000268.3
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UniProt Primary Accession #
P35240
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UniProt Secondary Accession #
O95683; Q8WUJ2; Q969N0; Q969Q3; Q96T30; Q96T31; Q96T32; Q96T33; Q9BTW3; Q9UNG9; Q9UNH3[Other Products]
UniProt Related Accession #
P35240[Other Products]
Molecular Weight
24,515 Da
NCBI Official Full Name
merlin isoform 1
NCBI Official Synonym Full Names
neurofibromin 2
NCBI Official Symbol
NF2 [Similar Products]
NCBI Official Synonym Symbols
ACN; SCH; BANF
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NCBI Protein Information
merlin
UniProt Protein Name
Merlin
UniProt Synonym Protein Names
Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomerlin; Schwannomin
UniProt Gene Name
NF2 [Similar Products]
UniProt Synonym Gene Names
SCH [Similar Products]
NCBI Summary for MERL
This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in *****s, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]
UniProt Comments for MERL
Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
Product References and Citations for anti-MERL antibody
Guang-Hui Xiao, et, al. (2005) Mol. Cell. Biol; 25: 2384-2394. Hi-Su Yang, et, al. (2006) Cancer Res; 66: 2708-2715. R Bohni, et, al. (1994) J. Biol. Chem; 269: 14541-14545. Adam J. Ratner, et, al. (2001) J. Biol. Chem; 276: 19267-19275.
Research Articles on MERL
1. The genetic alterations observed in the NF2 gene indicated that spinal schwannomas are associated with genetic alterations also found in other schwannomas and type 2 Neurofibromatosis, which reinforces the etiological role of this gene.
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