Notch2, Polyclonal Antibody

Notch2 Rabbit pab Antibody

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 1; NC_000001.10 (120454176..120612317, complement). Location: 1p13-p11

Polyclonal

IgG

Rabbit

Affinity purified

Liquid

1mg/ml (lot specific)

Upon delivery aliquot and store at -20 degree C or -80 degree C

Small volumes of anti-Notch2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. Notch2 is a member of Notch family and mutation in Notch2 is associated with Alagille syndrome (5).

Western Blot (WB)

WB: 1/500-1000

Western blot analysis of extracts of various cell lines, using NOTCH2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human stomach using NOTCH2 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using NOTCH2 antibody at dilution of 1:100 (40x lens).

265,405 Da

notch 2

neurogenic locus notch homolog protein 2; Notch homolog 2

Neurogenic locus notch homolog protein 2

Notch 2; hN2  [Similar Products]

NOTC2_HUMAN

1. Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
3. Schroeter, E.H. et al. (1998) Nature 393, 382-6.
4. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
5. McDaniell, R. et al. (2006) Am J Hum Genet 79, 169-73.

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