Merlin, Polyclonal Antibody

Anti -Merlin, phosphorylated (Ser518) (Schwannomin, CN, BANF, SCH, Neurofibromin 2, Schwannomerlin, Neurofibromatosis-2, NF2)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 22; NC_000022.10 (29999545..30094589). Location: 22q12.2

Polyclonal

Rabbit

Recognizes Human Neurofibromin 2 (NF2) when phosphorylated at Ser518. Species Crossreactivity: Mouse.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-NF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Na+/ H+ exchanger regulatory factor (NHERF1) is a PDZ domain containing adaptor protein known to bind to various receptors, channels, cytoskeletal elements and cytoplasmic signaling proteins. A 358aa mainly expressed in kidney, liver and pancrease by NHERF gene (chrm 17). And is represented in another isoform, NHERF2.

NHERF1 protein contains 2 tandem PDZ domains of approximately 90aa and a C-terminal sequence that binds several members of the ERM (ezrin-radixin-moesin) family of membrane cytoskeletal adapters. The protein plays an important role in regulation of NHE3, turnover of G-protein coupled receptors, platelet derived growth factor receptor and ion transporters such as CFTR, Na/Pi cotransporter, NaHCO3 cotransporter and Trp channels.
NHERF1 overexpression in cancers and mutations in NHERF1 targets, Merlin, the product of tumor suppressor gene, also expressed in 2 isoforms, Merlin is involved in pathogenesis of benign tumors of the human nervous system, Merlin is named for its striking similarity with ERM family which thought to link cytoskeletal components with proteins in the cell membrane, It has a binding partner called Syntenin, which is an adapter protein that couples transmembrane proteoglycans to cytoskeletal components.
Merlin, Isoforms 1 and 2 are predominant, isoforms 4, 5 and 6 are expressed moderately, and isoform 8 is found at low frequency. Isoforms 7, 9 and 10 are not expressed in ***** tissues, isoforms 1& 2 are 595aa and 590aa each in human, from same gene locus on chromosome 22q12, with approximate mol. wt of 70kD, they are structurally related to moesin, ezrin and redixin but not functionally. The Merlin isoforms are widely expressed in heart, lungs, skeletal muscle and spleen. Since being a membrane stabilizing protein, defects may lead to neurofibromatosis 2 a genetic disorder characterized by bilateral vestibular schwannomas. Affected individuals generally develop symptoms of eighth-nerve dysfunction in early *****hood, including deafness and balance disorder.

Antibodies; Abs to Disease Markers

Immunohistochemistry (IHC)

Suitable for use in Immunohistochemistry.

69,690 Da[Similar Products]

neurofibromin 2 (merlin)

merlin; schwannomin; schwannomerlin; neurofibromin-2; moesin-ezrin-radixin like; moesin-ezrin-radixin-like protein; moesin-ezrin-radizin-like protein; neurofibromin 2 (bilateral acoustic neuroma)

Merlin

SCH  [Similar Products]

MERL_HUMAN

This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in *****s, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]

Merlin: a moesin-ezrin-radizin-like protein. Interacts with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. Expressed at high levels during embryonic development; in *****s, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations are associated with neurofibromatosis type II. Two predominant isoforms and eight minor isoforms are produced by alternatively spliced transcripts.

Protein type: Tumor suppressor; Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: filopodium membrane; cortical actin cytoskeleton; early endosome; extrinsic to membrane; adherens junction; cytoskeleton; membrane; lamellipodium; perinuclear region of cytoplasm; apical part of cell; cytoplasm; nucleolus; plasma membrane; nucleus; cleavage furrow

Molecular Function: protein binding; actin binding

Biological Process: intercellular junction assembly and maintenance; negative regulation of MAPKKK cascade; hippocampus development; regulation of protein stability; negative regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of cell-matrix adhesion; odontogenesis of dentine-containing teeth; negative regulation of cell proliferation; Schwann cell proliferation; mesoderm formation; negative regulation of DNA replication; positive regulation of stress fiber formation; negative regulation of tyrosine phosphorylation of Stat3 protein; ectoderm development; negative regulation of protein kinase activity; negative regulation of cell-cell adhesion; positive regulation of cell differentiation; actin cytoskeleton organization and biogenesis; negative regulation of cell migration; negative regulation of JAK-STAT cascade

Disease: Meningioma, Familial, Susceptibility To; Neurofibromatosis, Type Ii; Schwannomatosis 1

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