BUB1B / BubR1, Monoclonal Antibody

Anti-BUB1B / BubR1 Antibody (clone 3F2) IHC-plus; BUB1B; Bub1A; BUBR1; HBUBR1; Protein SSK1; SSK1; BUB1beta; MAD3L; Human BUB1B; BubR1

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG,k

3F2

Mouse

Protein A Purified

PBS, pH 7.2

Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Small volumes of anti-BUB1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Subfamily:-BUB1">Family: Protein Kinase
Subfamily: BUB1

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

ELISA, IHC-P (10 ug/ml), WB
Usage: Western Blot (Cell lysate) - positive control Hela NE. Sandwich ELISA (Recombinant protein).

Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

BUB1B monoclonal antibody, clone 3F2 Western blot of BUB1B expression in HeLa NE.

Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.

121,386 Da

BUB1 mitotic checkpoint serine/threonine kinase B

mitotic checkpoint serine/threonine-protein kinase BUB1 beta; mitotic checkpoint kinase MAD3L; MAD3/BUB1-related protein kinase; budding uninhibited by benzimidazoles 1 homolog beta

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta

BUBR1; MAD3L; SSK1; hBUBR1  [Similar Products]

BUB1B_HUMAN

This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008]

BUB1B: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with CASC5. Induced during mitosis. Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. Kinase activity stimulated by CENPE. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Tumor suppressor; EC 2.7.11.1; Protein kinase, Other; Other group; BUB family

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: kinetochore; anaphase-promoting complex; perinuclear region of cytoplasm; cytoplasm; microtubule organizing center; outer kinetochore of condensed chromosome; spindle midzone; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: mitosis; cell proliferation; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; apoptosis; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; protein amino acid phosphorylation; mitotic metaphase/anaphase transition

Disease: Mosaic Variegated Aneuploidy Syndrome 1; Premature Chromatid Separation Trait

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