Sodium/potassium-transporting ATPase subunit alpha-3 (ATP1A3), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ATP1A3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7252834 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sodium/potassium-transporting ATPase subunit alpha-3 (ATP1A3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATP1A3. The ELISA analytical biochemical technique of the MBS7252834 kit is based on ATP1A3 antibody-ATP1A3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATP1A3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATP1A3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Neuroscience

113,059 Da

ATPase, Na+/K+ transporting, alpha 3 polypeptide

sodium/potassium-transporting ATPase subunit alpha-3; Na+/K+ ATPase 3; sodium pump subunit alpha-3; Na(+)/K(+) ATPase alpha-3 subunit; Na(+)/K(+) ATPase alpha(III) subunit; sodium-potassium-ATPase, alpha 3 polypeptide; sodium-potassium ATPase catalytic subunit alpha-3; sodium/potassium-transporting ATPase alpha-3 chain

Sodium/potassium-transporting ATPase subunit alpha-3

Na(+)/K(+) ATPase alpha-3 subunit  [Similar Products]

AT1A3_HUMAN

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

ATP1A3: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Defects in ATP1A3 are the cause of dystonia type 12 (DYT12); also known as rapid-onset dystonia parkinsonism (RDP). DYT12 is an autosomal dominant dystonia- parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT12 patients develop dystonia and parkinsonism between 15 and 45 years of age. The disease is characterized by an unusually rapid evolution of signs and symptoms. The sudden onset of symptoms over hours to a few weeks, often associated with physical or emotional stress, suggests a trigger initiating a nervous system insult resulting in permanent neurologic disability. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

Protein type: Membrane protein, multi-pass; Transporter, ion channel; Transporter; EC 3.6.3.9; Endoplasmic reticulum; Membrane protein, integral; Hydrolase

Chromosomal Location of Human Ortholog: 19q13.31

Cellular Component: Golgi apparatus; endoplasmic reticulum; axon; integral to membrane; plasma membrane; synapse; nucleus; sodium:potassium-exchanging ATPase complex; vesicle; myelin sheath

Molecular Function: metal ion binding; chaperone binding; sodium:potassium-exchanging ATPase activity; D1 dopamine receptor binding; ATP binding

Biological Process: response to drug; cellular sodium ion homeostasis; potassium ion import; metabolic process; ionotropic glutamate receptor signaling pathway; ***** locomotory behavior; visual learning; transmembrane transport; cellular potassium ion homeostasis; memory

Disease: Dystonia 12; Cerebellar Ataxia, Areflexia, Pes Cavus, Optic Atrophy, And Sensorineural Hearing Loss; Alternating Hemiplegia Of Childhood 2

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